A rapid and label-free DNA amplification/detection technique using combination of helicase DNA amplification (HDA) and biophotonic sensor complex

7^th International Conference and Expo on Molecular & Cancer Biomarkers

September 15-16, 2016 Berlin, Germany

Yong Shin

University of Ulsan College of Medicine - Asan Medical Center, Korea

Posters & Accepted Abstracts: J Mol Biomark Diagn

Abstract :

Epidermal growth factor receptor (EGFR) is a non-small cell lung cancer biomarker used to predict response of treatment on individual patient by using near patient testing method. Existing methods for detection of EGFR mutation status are costly, labor- and time-intensive. Here, we report an Isothermal DNA amplification/detection technique based on Helicase DNA Amplification (HDA) and Silicon Microring Resonator complex, designated detection of EGFR mutation for near patient testing in non-small cell lung cancer (NSCLC) patient. We combined a HDA assay as an isothermal DNA amplification technique and a biophotonic sensor based on silicon microring resonators to create a sensing platform for label-free and real-time monitoring of DNA amplification and detection. Moreover, complex thermal components are not necessary for this device because of the use of the HDA assay. The system delivers a result in 45 min and was able to detect a L858R mutation in a sample containing only 10% of the mutant cells in a mixture of wild-type cells compared to conventional sequencing method (>30%). Therefore, this complex system can be useful for the fast detection of L858R mutation of EGFR gene in clinical samples and this will ensure that the clinician gives appropriate treatment to patient in clinician�s office.

Biography :

Email: shinyongno1@gmail.com