A rapid and sensitive detection of glutathione using nanostructured V2O5 mimicking the oxidase activity as inorganic nanozyme

Joint Event on 2^nd Edition of Graphene & Semiconductors | Diamond Graphite & Carbon Materials Conference & 6^th Edition of Smart Materials & Structures Conference

April 16-17, 2018 Las Vegas, Nevada, USA

Akhilesh Babu Ganganboina and Ruey-an Doong

National Tsing Hua University, Taiwan
National Chiao Tung University, Taiwan

Posters & Accepted Abstracts: J Material Sci Eng

Abstract :

Glutathione detection has been one of the important and critical line of investigations for biotechnologists in recent years. Herein, we introduce vanadium pentoxide nanosheets (V2O5 NS) as a novel nanozyme mimicking peroxidase reaction as a fast selective colorimetric assay for the detection and quantification of glutathione (GSH). The V2O5 NS have been prepared by ultrasonication assisted exfoliation of bulk V2O5 and characterised. The present process involves catalysis by V2O5 in the oxidation of pale yellow 3,3ʹ,5,5ʹ,-tetramethylbenzidine (TMB) to blue color with an absorption peak centered at 650 nm. On introduction of GSH, a fading in deep blue color of oxidized TMB occurs with a simultaneous decrease in absorbance intensity at 650 nm, indicating the sensitivity of V2O5 catalysed reaction. Also, GSH selectively inhibits this reaction with a detection limit of 10 nM. The high specificity of inhibition by GSH allows this system to be used for determination of GSH concentration in human serum samples. This method is simple, fast and cost effective and can evolve as a potential method in discriminatively detecting GSH. ganganboinaakhilesh89@gmail.com