Kabir, Mohammad Humayun; Hasan, Nur; Rahman, Md. Mahfuzur; Rahman, Md. Ashikur; Khan, Jakia Alam; Hoque, Nazia Tasnim; Bhuiyan, Md. Ruhul Quddus; Mou, Sadia Moin; Jahan, Rownak; Rahmatullah and Mohammed
Posters-Accepted Abstracts: J Tissue Sci Eng
The importance of fish cell culture work has got momentum as because of potential applications of fish cell lines in areas like fish pathology and immunology, toxicology, endocrinology, virology, biomedical research, biotechnology and radiation and developmental biology. Fish cell lines have been utilized as a rapid and cost-effective versatile tool in toxicological assessment of aquatic pollutants. The number of fish cell lines has been increasing tremendously covering a wide variety of species and tissues of origin during last decades. At present, more than fifty fish cell lines have been developed in India covering a wide variety of species and tissues of origin. This has raised the need of conserving the cell lines in one secured place. NBFGR, Lucknow is the state of art facility for development, characterization and storage of cell lines. A National repository of fish cell line (NRFC) has been established at National Bureau of Fish Genetic Resources (NBFGR) with the financial assistance from Department of Biotechnology (DBT), Govt. of India. At present, 50 fish cell lines from 24 different fish species are being maintained and cryopreserved in the NRFC. These cell lines were deposited by various research groups working on fish cell lines including the researchers at NBFGR. The cell lines were authenticated and characterized using DNA barcodes, karyotypes and protein expression signatures. The paper highlights different aspects related to characterization and applications of fish cell lines for fish germplasm conservation.
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