Albuminuria-induced violations in apoptosis controlling system in children with nephrotic syndrome

International Conference and Exhibition on Molecular Medicine and Diagnostics

August 24-26, 2015 London, UK

Maidannyk V and Burlaka Ie

Posters-Accepted Abstracts: J Mol Genet Med

Abstract :

Proteinuria not only is a sign of kidney damage, but is also involved in the progression of renal diseases as an independent
pathologic factor. Clinically, glomerular proteinuria which is the most commonly observed, relates to structural and functional
anomalies in the glomerular filtration barrier. The objective of this project was to study the markers of apoptosis in kidney tissue in
children with chronic glomerular diseases. 32 patients aged 5-18 years with an active stage of nephrotic syndrome were included to
the study. Immunohistochemical examination of proapoptotic factor Bax, antiapoptotic factor Bcl-xL levels, apoptosis evaluation
in kidney biopsy specimens was done. Analysis of the level of proapoptotic factor Bax in kidney slices obtained from children with
morphological form focal segmental glomerulosclerosis (FSGS) showed the presence of high levels of Bax in both glomerular and
tubular-interstitial segments. However, higher immunosignal was recorded in glomeruli with FSGS I-II st. compared to tubular
segment. When complete glomerular sclerosis was observed, high levels of Bax were observed in the surrounding tubuli and
interstitial segment. In kidney tissue of nephrotic patients, presence of a certain level Bcl-xL in glomeruli, tubuli and interstitium was
found. Higher immunosignal was recorded in tubuli, interstitial segment compared to glomeruli with FSGS I-II st. When complete
glomerular sclerosis occurs, relatively high immunosignal of Bcl-xL is localized in the surrounding tubuli, interstitial segment with
almost complete absence in glomeruli. Quantitative analysis of apoptosis levels in kidney sections of patients with nephrotic syndrome
and FSGS I-II st. revealed apoptotic index (AI) in glomeruli at level 21.5±0.9%, in the tubuli and interstitial component – 9.12±0.34%
(p<0.01). With FSGS III-IV st. high AI was found in tubuli, interstitial component – 31.22±1.14%, in the glomeruli – 4.15± 0.6%
(p<0.001). Thus, progression of glomerulosclerosis as an irreversible kidney damage induced by chronic albuminuria is associated
with increased activity of proapoptotic factor Bax and simultaneous reduction of anti-apoptotic factor Bcl-xL. The manner of Bax and
Bcl-xL distribution in relation to the stages of FSGS is an indicator of step-dependent manner of glomerular and interstitial injuries
development under the influence of proteinuria.

Biography :

Ievgeniia Burlaka, MD, PhD has completed PhD in 2009 in National O.O. Bogomolets Medical University (Kyiv, Ukraine). In 2008-2014 worked as a researcher in Karolinska
Institutet (Sweden, Stockholm) in Department of Children’s and Women’s Health in Molecular and Cellular Pediatric Laboratory.
Currently works as a Professor’s Assistant in Department of Pediatrics âÂ?Â?4 in National O.O. Bogomolets Medical University (Kyiv, Ukraine). Current research interest is
studying of the initial molecular disorders leading to the irreversible kidney damage in patients with albuminuric kidney diseases. She has published more than 60 papers
in international and local Ukrainian journals.