Animal and cellular models of hepatocellular carcinoma bone metastasis: Establishment and characterization

10^th Global Annual Oncologists Meeting

July 11-13, 2016 Cologne, Germany

Rui Hou

Peking Union Medical College Hospital, China

Posters & Accepted Abstracts: J Cancer Sci Ther

Abstract :

An increasingly high occurrence of bone metastases in Hepatocellular Carcinoma (HCC) patients highlights the importance of fundamental research on HCC bone metastasis, which has been limited in its success due to the lack of a model system. We successfully established a nude mouse model of HCC bone metastasis using the HCC cell lines HCCLM3 and MHCC97H via intracardiac injection. Injected tumour cells formed metastases in skull, spine, hind limbs, and sternum, causing osteolytic lesions via MMP-1 and recruitment of osteoclasts. Four bone metastatic cell lines (BM1) were extracted from HCCLM3-inoculated mice and were demonstrated to exhibit a much stronger ability to form bone metastases as well as other phenotypes, including enhanced in vitro migration/invasion and colony formation. Moreover, the expression of PTHrP, MMP-1, and CTGF was significantly elevated in bone metastatic cells compared to parental cells. In conclusion, the nude mouse model and bone metastatic cell lines (BM1) together provide an effective simulation of HCC bone metastasis. These model systems will become powerful tools with which to explore the mechanisms and therapies of HCC bone metastasis. Additionally, PTHrP, MMP-1, and CTGF are candidate genes related to HCC bone metastasis.

Biography :

Email: henrequelme@aliyun.com