Antibacterial activity of biogenic silver nanoparticles against Salmonella enterica

Joint Conference International Congress on Nosocomial and Healthcare Associated Infections & 2^nd Global Medical Microbiology Summit & Expo

October 02-04, 2017 Las Vegas, USA

Meiriele S Neves, Sara Scandorieiro, Renata K T Kobayashi and Gerson Nakazato

Londrina State University, Brazil

Scientific Tracks Abstracts: J Med Microb Diagn

Abstract :

The increase of resistant strains is a major problem in these days. Researchers are testing new antimicrobials, inclusive compounds produced by nanotechnology. Salmonella is a genus of bacteria that are a major cause of foodborne illness in the world and are transmitted through contaminated food. In this study, we tested biologically synthesized silver nanoparticles (AgNPbio) against Salmonella enterica. AgNPbio were prepared according to a method by Dur�¡n et al., 2005. The AgNPbio diameter and zeta potential were determined by photon correlation spectroscopy. Minimal inhibitory concentrations (MICs) were determined by broth microdilution assays in 96-well plates, as suggested by CLSI. The in vitro antibacterial activity of AgNPbio was examined against four reference bacterial strains (ATCC), Staphylococcus aureus 25923, Escherichia coli 25922, Salmonella typhimuirum UK1 and Salmonella enteritidis 13076, and 19 bacteria isolated from chicken. Minimal bactericidal concentration (MBC) was determined by sub-culturing 10 �¼L from the broth dilution MIC. The AgNPbio presented the size range 81.25 nm, zeta potential â��36.4 mV and PI= 0.296. E. coli and S. aureus with MIC of 39.4 �¼M while S. typhimuirum and S. enteritidis showed MIC value of 78.7 �¼M. Isolates from chicken also showed sensitivity for AgNPbio with MIC ranged from 78.7 �¼M to 157.5 �¼M, and all bacterial strains showed the MBCâ�¤157.5 �¼M. The AgNPbio is a great alternative because it was not related to any bacterial strain naturally resistant to silver nanoparticles. Our study suggests that the use of AgNPbio can be effective against Salmonella enterica strains, an important food pathogen.

Biography :

Meiriele S Neves is a PhD student from Londrina State University, Brazil. She has studied about natural antimicrobials against Salmonella enterica. She works with biological silver nanoparticle obtained from Fusarium oxysporum and oregano oil. This study can help to develop alternatives to control food contamination. Her interest includes application of AgNPbio in food products and the interaction of this compound with the food.