Antibacterial properties of new bacterium isolated from date palm leaves affected by brittle leaf disease

5^th International Conference and Exhibition on Pharmaceutical Regulatory Affairs

August 03-05, 2015 Orlando, USA

Mouna Jrad1, Ben Choba Ines1, Ben Bacha Abir1,2, Drira Nourredine1 and Gharsallah N�©ji1

1University of Sfax, Tunisia 2King Saud University, Saudi Arabia

Posters-Accepted Abstracts: Pharmaceut Reg Affairs

Abstract :

An actinomycete strain was isolated from date palm leaves affected by brittle leaf disease (Deglet Nour, Tunisia). The strain was classified as a new bacterium, an endophytic strain. Identification was based primarily on the morphological characteristics. This strain was grown on a medium rich tryptic soy broth (TSB) with K2HPO4 (1 mM) and MgSO4, 7H2O (2 mM) at 28�°C and neutral pH. It is a Gram-positive filamentous bacterium with broad spectrum antimicrobial activity. The optimum pH for the product was 7 to 7.4. The highest product yield was with glucose and tryptone at 1% (w/v) as carbon and nitrogen sources, respectively. Incubation between 25�°C and 30�°C for 24 hours was optimal for the bioactive metabolite(s) production. The product remained stable up to 50 hours. The bioactive metabolite(s) produced by endophytic bacteria was partially purified and studied for antibacterial characteristics using the wells technique. Analysis by bioautography and gas chromatography/mass spectroscopy (GC/MS) of the supernatant from precipitation with 100% ammonium sulphate indicated that the strain produced a high percentage of fatty acid. Additionally, a cyclic compound could be involved in the antibacterial activity. After silica plate scraping, bioactivity disappeared at 70�°C. Bioactive molecule(s) may be involved in the brittle leaf disease, because its product isolated by a strain from date palm leaves is affected by the brittle leaf disease enough. In addition, this endophytic has shown best lipolytic activity and produced a lipidic biomolecule(s), this result explains the implication of metabolite(s) in the brittle leaf disease. Finally, determination of MIC and MBC of bioactive molecule(s) against strains (Bt, Bs).

Biography :

Email: jradmouna@yahoo.com