Antibiotic or a phage? Evaluation of eradication methods of Salmonella-derived biofilms under different growth conditions in laboratory environment and potential applications

Joint Event on 14^th International Conference on Microbial Interactions & Microbial Ecology & 11^th Edition of International Conference on Advances in Microbiology and Public Health

August 19-20, 2019 Vienna, Austria

Katarzyna Kosznik-Kwasnicka, Lukasz Grabowski, Monika Sikora, Aleksandra Dydecka, Alicja Nowak-Zaleska and Alicja Wegrzyn

Institute Biochemistry and Biophysics, Polish Academy of Sciences, Poland
University of Gdansk, Poland

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

Salmonella enterica is one of the most common foodborne pathogens associated with contaminated poultry meat and eggs. The genus is subdivided into over 2500 serovars, with different level of pathogenicity. Most common serotypes responsible for the disease in humans are S. Typhimurium and S. Enteritidis. S. enterica commonly live in gastrointestinal tract of farming birds without causing any illness symptoms. However, any damage done to birdsâ?? intestines during meat processing may result in bacteria spreading onto the meat and also onto the equipment in the abattoir. S. enterica is able to form biofilm on various surfaces, and thus it can be more resistant to different methods of eradication. In our work, we have analysed the formation of biofilms of S. enterica serotypes commonly associated with salmonellosis in different temperatures, under laboratory conditions. We have also compared the effectiveness of antibiotics, disinfectant and bacteriophages in eradication of biofilms formed by those serotypes under different growth conditions. Moreover, we have analysed the effectiveness of phages and antibiotics against S. enterica in multi-species biofilm in order to compare therapeutic potential of phage therapy as an alternative to antibiotics. We have observed that bacteriophages tended to be equally effective as antibiotics in eradication of S. enterica biofilms formed under laboratory conditions. However, we have noticed that reapplying phages on previously treated biofilm did not cause its further decomposition. Therefore more research needs to be conducted in order to evaluate the potential of phages as an alternative treatment of Salmonella-derived biofilms.

Biography :

E-mail: katarzyna.kwasnicka@biol.ug.edu.pl