Antibiotic susceptibility pattern of Salmonella and Pseudomonas species isolated from meat market and Ogoja road abattoir effluents in Abakaliki Metropolis

9^th World Congress and Expo on Applied Microbiology

October 25, 2021 WEBINAR

T.E. Ayogu, J.O. Orji, E.C. Nwojiji, R.C. Umezurike and Ude Ude Ibiam

Department of Microbiology Ebonyi State University Abakaliki, Nigeria

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

This work was aimed at determining the antibiotics susceptibility patterns of Salmonella and Pseudomonas species isolated from abattoir effluents in Abakaliki Metropolis. A total of thirty (30) abattoir effluent samples (5 from each collection points viz; butchering point, rinsing point and discharging point) were collected and transported to the microbiological laboratory unit of Ebonyi State University for bacteriological analysis. Bacteria isolated were characterized using standard microbiological and biochemical techniques. A total of fifty (50) bacterial isolates (27 from meat market abattoir and 23 from Ogoja road abattoir) were isolated from both meat market and Ogoja road abattoirs. Out of the 50 bacterial isolates, 30 (60 %) were Salmonella species while 20 (40 %) were Pseudomonas species. A total of 15 Salmonella species and 12 Pseudomonas species were isolated from meat market abattoir, while a total of 15 Salmonella species and 8 Pseudomonas species were isolated from Ogoja road abattoir. Antibiotic susceptibility study on the organisms was carried out using Kirby Bauer disc diffusion method according to Clinical Laboratory Standards Institute, (CLSI, 2005). The Salmonella species were most sensitive to gentamicin (90 %), followed by meropenem (80 %), ceftriaxone (50 %), ofloxacin (30 %), cefuroxime (20 %) and caftazidime (10 %) while amoxycillin (0.0 %), cefotaxime (0.0 %) and clindamycin (0.0 %) showed the least activity against the bacteria. Pseudomonas species were more susceptible to meropenem (80 %), followed by gentamicin; (80 %), ofloxacin (60 %), ceftriaxone (20 %), ciprofloxacin (20 %), cefuroxime (20 %) and amoxycillin (20 %) while erythromycin (0 %), ceftazidime (0 %) cefotaxime (0 %) and chindamycin showed the least. The multiple antibiotics resistance (MAR) index of Salmonella was 0.73 from the two abattoirs while that of the Pseudomonas from meat market abattoir was 0.79 while that from Ogoja road was 0.82. This gave an indirect suggestion of the probable source(s) of the organisms. The presence of these multidrug resistant bacterial strains isolated from abattoir effluents could be a vehicle for the spread of antibiotic resistance gene to other bacteria. Hence, there is need for adequate treatment and safe disposal of abattoir effluent in Ebonyi State and also in Nigeria at large.