Antimicrobial resistance of Brucella isolated from seropositives cattle in the department of Tizi Ouzou, Algeria

47^th World Congress on Microbiology

September 10-11, 2018 | London, UK

Nedjma Lounes, Abdallah Bouyoucef, Malika Lazri, Nabila Benamrouche, Hassiba Tali Maamar and Kheira Rahal

National Veterinary High School (ENSV), Algiers, Algeria
Medical Bacteriology Laboratory, Pasteur Institute of Algeria, Algiers, Algeria

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

Bovine Brucellosis is enzootic and widespread in all departments of Algeria, with variable prevalence depending on the region. During the two last decades, the veterinary authorities declared an average infection rate of 1 % in cattle. All the investigations and epidemiological data were based only on serological surveys, and didn�t provide the knowledge on the epidemiology of Brucella circulating strains. The epidemiological map of prevailing species and biotypes of Brucella in Algerian livestock have never been established. Our study aims (i) to isolate and to identify the different biotypes of Brucella prevailing in the department of Tizi Ouzou (centre north Algeria) from cattle, and (ii) to study their susceptibility to antibiotics. Between October 2011 and May 2014, 32 samples, including 14 milks and 18 lymph tissues (9 retropharyngeal and 9 supramammary lymph nodes) were collected from 15 infected cattle (detected during screening and slaughtered under the control program) coming from 11 farms, situated in six town of the department. Brucella strain characterization were performed according to the technique described by the French standard AFNOR NF U47-105. The sensitivity of the isolated strains to six antibiotics (streptomycin, rifampicin, gentamicin, tetracycline, doxycycline and trimethoprimsulfamethoxazole) was tested by E-test method. A total of 11 strains of Brucella were isolated, 3 from milks (27%) and 8 from lymph nodes (73%), 3 from retropharyngeal (27%) and 5 from supramammary (45,5%). All isolated strains was classified to Brucella abortus biovar 3. The E-test revealed that 4 of the isolated strains were resistant to streptomycin (36,4%). This study represents the first investigation in Algeria on the characterisation of Brucella strains from cattle. This results may contribute (i) to establish the epidemiological map of the distribution of different Brucella strains prevailing in the this region, and (ii) alert to the existence of the antimicrobial resistance of Brucella.

Biography :

E-mail: lounesnedjma@gmail.com