Anti-myocardial ischaemic effect of pseudo-ginsenoside GQ injection against beta1-adrenoceptor (β1- AR) in rat cardiac myocytes (H9c2)

9^th Annual European Pharma Congress

June 26-28, 2017 Madrid, Spain

Ping-Ya Li, Jin-Ping Liu, Cui-Zhu Wang and Nan-Qi Zhang

Jilin University, China

Posters & Accepted Abstracts: Pharmaceut Reg Affairs

Abstract :

Statement of the Problem: Myocardial ischemia (MI) is a prevalent complicated heart disorder worldwide. It is a clinically common pathophysiological phenomenon which characterized by reduced blood supply to the heart, resulting in nutrients depletion and causing hypoxia. Although there are many precious researches on MI injury, it is perennial among the leading causes of morbidity and mortality in humans in the industrialized countries. Therefore, research of an efficient agent for MI injury remains an important issue. Pseudo-ginsenoside GQ (PGQ) injection (1.1 class chemicals), firstly developed by our lab., is based on PGQ obtained by modifying 20(S)-ginsenoside Rg3 with the biological degradation and the chiral semi-synthesis method, etc., which was approved by the Chinese State Food and Drug Administration for the treatment of myocardial ischemia and phase I trial was carried on to evaluate the safety of PGQ injection. Methodology & Theoretical Orientation: The effects of PGQ injection on the cardiovascular physiology of MI injury rats were examined by measuring various electrocardiographic parameters. NBT staining method was utilized to measure the heart infarct size, and the levels of LDH, CK, SOD, AST, MDA and cTnT were measured to further evaluate and validate the protective function of PGQ injection during MI injury. Flow cytometry and western blot analysis were used to detect the �²1-AR expression in vitro. Findings: Both data and histopathological examination all demonstrated that PGQ injection could significantly improve the heart function and decrease infarct size. And the levels of CK, LDH, AST, MDA and cTnT were decreased and the activities of SOD were increased. The underlying mechanism was explored by flow cytometry and western blot, and results showed that the expression of �²1-AR was decreased by PGQ injection. Conclusion & Significance: This study provides the substantial evidence for the effect of PGQ injection on myocardial ischemia.

Biography :

Email: lipy@jlu.edu.cn