Antitumor, antioxidant, antimicrobial activities of the methanolic extract of licorice roots

3^rd International Conference on Pharmaceutical Regulatory Affairs

October 21-23, 2013 DoubleTree by Hilton Hotel San Francisco Airport, CA, USA

Amani M. D. El-Mesallamy, Mohamed El-Gerby, Mohamed H. M. Abd El Azim and Ahmed Awad

Posters: Pharmaceut Reg Affairs

Abstract :

Twenty phenolic compounds were isolated from the methanolic extract of licorice roots (Glycyrrhizia glabra L.) and identified on the basis of chromatographic methods, HPLC and spectroscopic data. The efficiency of licorice methanolic extract as anticancer agent for breast, colon and liver was tested. The results showed that the IC50 of the extract was 28 μg/ml for anticolon cancer and 31.2 μg/ml for antibreast cancer, while it was 3.43 μg/ml for antihepatic cancer. The antioxidant activity was measured by 2, 2'-Diphenyl Picrylhydrazyl (DPPH) radical scavenging method at three different concentrations0- 25, 50, 100 μl. This extract showed strong antioxidant activity against DPPH as compared with vitamin C. Antimicrobial activity of methanolic extract of licorice was studied against three bacterial strains (E. coli, Staphyllococcus aureus and Salmonella typhi) and four fungal species (Fusarium oxysporum, Aspergillus niger, Penicillium sp. and Trichoderma sp.) at concentration 0.1 ml and 0.3 ml (10 mg / 1 ml). The extract showed strong inhibitory effect for most species at concentration 0.3 ml (10 mg / 1 ml). Keywords: Licorice, Glycyrrhiza glabra, Phenolic constituents, Antitumor, Antioxidant, Antimicrobial