Anti-wrinkle activity of the ethanolic extracts of the leaves of Morus alba L. and Morus nigra L.

3^rd International Conference and Exhibition on Traditional & Alternative Medicine

August 03-05, 2015 Birmingham, UK

Mai M Raslan1, Maha M Salama2 and Shahira M Ezzat2

1Beni-Suef University, Egypt 2Cairo University, Egypt

Posters-Accepted Abstracts: Altern Integr Med

Abstract :

UV irradiation damages skin cells by the photochemical generation of reactive oxygen species (ROS) that damages nucleic acids, lipids, and proteins, including collagen. UVB light is mainly absorbed in the epidermis, which impairs the synthesis of collagen and induces matrix metalloproteinases (MMPs), and hence contributes to wrinkle formation. Antioxidants protect skin from free radicals, thus fighting the signs of aging. The antioxidant capacity of the ethanolic extract of the leaves of Morus alba and Morus nigra was evaluated applying: 2,2-diphenyl-1-picryl-hydrazyl (DPPH), 2,2`-azino-bis (3-ethylbenzo-thiazoline- 6-sulfonic acid), diammonium salt (ABTSâ�¢+), ferric reducing antioxidant power assay and ferrous ion-chelating assay. The antiwrinkle activity was assessed by subcutaneous administration of EAR/15weeks in UVB irradiated mice then measuring wrinkle score, MMP-2 & MMP-9 gene expression, in addition to, measuring skin thickness and elasticity histologically. M. alba and M. nigra leaves showed high free radical scavenging activity against DPPH with IC50 494 and 726 �¼g/ml, respectively, and against ABTSâ�¢+ with IC50 765.69 and 897.66 �¼g/ml, respectively, both leaves also showed high Fe3+ and Fe2+ chelating ability. Antiwrinkle results revealed that M. alba and M. nigra leaves inhibited MMP-2 expression (0.6�±0.04 and 0.54�±0.08, respectively), MMP-9 (0.78�±0.06 and 0.68�±0.08, respectively) and low wrinkle score (2.65�±0.42 and 2.44�±0.48, respectively), these results were significant with respect to the diseased group which showed 0.72�±0.08, 1.28�± 0.2 and 3.70�±0.23 for MMP-2, MMP-9 and score wrinkles. The metabolic profile of the ethanolic extracts of both leaves was studied using LC-MS technique. The high antioxidant activity of the standardized EAR is responsible for its anti-wrinkle activity and hence could be incorporated in skin formulations, based on its antioxidant activity.

Biography :

Mai M Raslan has completed her PhD in 2011 from Cairo University, Faculty of Pharmacy, Egypt. She is the Director of Quality Assurance unit in Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Egypt.

Email: m2raslan@yahoo.com