Application of bone-marrow mesenchymal stem cells and platelet-derived growth factors for human osteogenic graft engineering

5^th International Conference on Tissue Engineering & Regenerative Medicine

September 12-14, 2016 Berlin, Germany

Nataliya Danilkovich, Victor Derkachev, Svetlana Kosmacheva and Michael Potapnev

Belarusian State Medical University, Belarus

Posters & Accepted Abstracts: J Tissue Sci Eng

Abstract :

Tissue engineering is one of the perspective approaches for therapy of large bone defects resulted from trauma or tumor resection and non-healing fractures. Composition of biograft includes collagen-hydroxyapatite scaffold, human mesenchymal stem cells (hMSCs), preparations of platelet-derived growth factors (PDGFs) and fibrin glue. The purpose of the study was to assess the effect of soluble preparations of PDGFs on hMSCs proliferation then osteogenic differentiation in vitro and to support bone reconstruction in vivo. Preparations of PDGFs were platelet releasate (PR) and platelet lysate (PL). In our experiments, in vitro PR and PL were shown to increase cell proliferation by 16.6- and 14.4- fold respectively at the final concentration of 5% compared to a basal medium with 10% fetal bovine serum. Induction of osteogenic differentiation of MSCs in vitro by �²-glycerophosphate, L-ascorbic acid and dexamethasone was up-regulated by PL (in 5% concentration), as it was demonstrated by augmentation of osteopontin (SSP1) gene expression for 4.7-fold and osteocalcin (BGLAP) gene expression for 18.9 fold. Experiments in vivo showed that rabbit radial bone defects of 1.5��0.7��0.3 cm in size were efficiently replaced by MSC-based osteogenic graft. X-ray densitometry showed the increase of bone density to 2200�±100 Hounsfield units (HU) in site of biograft implantation in comparison with 1800�±100 HU in non-treated control 3 months post operation. Immunohistochemical staining elicited osteogenesis activation with tubular bone formation in site of biograft replacement. Healing process was weaker when only pre-differentiated hMSCs and scaffold without PDGFs were used in biograft composition. To summarize, hMSCs, PL, collagen-hydroxyapatite scaffold and fibrin glue are the principal components in engineering of MSC-based human osteogenic graft for bone repair, healing and substitution.

Biography :

Nataliya Danilkovich has graduated from the Belarusian State University, Biological Faculty, Department of Microbiology from Belarus. Currently, she is a PhD candidate at the Department of Cellular Biotechnologies, The Republic Research Center for Transfusiology and Medical Biotechnologies. She has published 1 international and 13 national paper.

Email: nndanilkovich@gmail.com