Assessing role of SATB2 and SATB2-AS1 genes in Colorectal Cancer

International Conference on Euro Oncology, Breast Cancer & Biomarkers

October 18-19, 2018 | Amsterdam, Netherlands

Zi Yan Chiah, Camille Hurley, Ian Miller, Annette Byrne, Sudipto Das and Darran O Connor

RCSI Research Summer School, Ireland
Royal College of Surgeons, Ireland

Posters & Accepted Abstracts: J Cancer Sci Ther

Abstract :

SATB2-AS1 is an antisense transcript to SATB2, the latter is described as a well categorized tumour suppressor gene in colorectal cancer(CRC). Thus, it is plausible that SATB2-AS1 might play a role in CRC progression. This study was aimed to examine the expression levels of SATB2-AS1 both in vitro and in vivo. mRNA expression of SATB2 and SATB2-AS1 was assessed in SW620(metastatic) and SW480(benign) CRC cell line and in vivo orthotopic mice models(same cell line) treated with either FOLFOX alone, combination of FOLFOX and Avastin, Avastin alone or vehicle. RNA was extracted and used for cDNA synthesis followed by gene specific expression testing using quantitative real-time PCR(qRT-PCR). Endogenous levels of both SATB2 and SATB2-AS1 were higher in SW480 compared to SW620 cell line. SATB2 was elevated in SW620 combination treated samples, in SW480 FOLFOX alone treated sample and was lowered in SW480 combination treated samples in mice models relative to vehicle. Moreover, SATB2-AS1 was up-regulated in SW620 Avastin alone treated samples and in SW480 combination treated samples in derived mouse model relative to the vehicle. We also examined the potential silencing of the SW620 genes due to DNA methylation(5â??-aza-2â??-deoxycytidine treatment) compared to SW480. No significant alteration was found but both genes were significantly elevated relative to vehicle. Elevated SATB2 and SATB2-AS1 levels suggest a plausible protective role of both genes in CRC. In addition, we demonstrate that both SATB2 and SATB2-AS1 appear to be co-regulated by DNA methylation. Future studies will allow us to ascertain the precise role of this anti-sense transcript in CRC..

Biography :

Zi Yan Chiah is currently pursuing his third year undergraduate Medicine degree in Royal College of Surgeons in Ireland (RCSI). In summer of 2017, he was awarded funding from the RCSI Research Summer School and carried out his research on colorectal cancer supervised by Dr. Darran O’Connor and Dr. Sudipto Das.

E-mail: ZiYanChiah@rcsi.com