Manisha Mishra, Arora Pallavi, Saxena Shobhit, Gupta Sunil, Mochan Sankat, Kasapuram Dheeraj, Singh Seema, Bhatla Neerja,Sharma Arundhati, Dhingra Renu and Rani Neerja
All India Institute Of Medical Sciences, India
Scientific Tracks Abstracts: J Mol Genet Med
Background & Aim: Imbalanced Matrix Metalloproteinase (MMP) expression, including MMP-2, has been
demonstrated in pre-eclampsia. However, little is known about the effect of MMP-2 gene polymorphisms
on hypertensive disorders of pregnancy. Therefore, we examined Matrix Metalloproteinase (MMP-2) gene
polymorphisms (g.-735C/T) and its association with Preeclampsia (PE) and measured the levels of MMP-2
serum concentrations in PE.
Method: 30 preeclamptic and 30 healthy pregnant women were enrolled from department of obstetrics and
gynaecology, AIIMS, New Delhi after getting approval from Institute ethical committee. Genomic DNA
was extracted from blood and amplified by PCR. MMP2 gene polymorphisms of -735C/T was detected by
Restriction Fragment Length Polymorphism (RFLP). The levels of MMP2 in sera were measured by ELISA.
Result: The maternal serum MMP2 levels was found to be more in PE patients than in control group (p=0.03).
The increased frequency of CT genotype for MMP2 (-735C/T) Single Nucleotide Polymorphism was seen in
PE patients as compared to control group. However the difference in genotype frequency was not statistically
significant (p=0.35).
Conclusion: These findings may help to understand the relevance of MMP-2 and its genetic polymorphisms
to the pathophysiology of hypertensive disorders of pregnancy like preeclampsia and IUGR.
Manisha Mishra had completed Bachelor of Dental Surgery and MSc Anatomy from All India Institute of Medical Sciences, New Delhi, India. She is currently a Senior Reseach Fellow at All India Institute of Medical Sciences, India.
E-mail: mani040790@gmail.com
Molecular and Genetic Medicine received 3919 citations as per Google Scholar report
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