Beneficial Effects of Adipose-Derived-Mesenchymal Stem Cells (AD-MSCs) versus Anti-Parkinson drug in a rat model of Parkinsons disease: Relationship to the molecular genetic expressions, ultrastructural and physiological

Joint Event on 11^th International Conference on Tissue Engineering & Regenerative Medicine & 4^th International Conference on Synthetic Biology and Tissue Engineering

October 18-20, 2018 Rome, Italy

Naglaa K Idriss, Fatma Y. Meligy, Dalia A. Elgamal, Eman S.H. Abd Allah, Nagwa M. Ghandour, Ehab R. Biomy, Azza Sayed Abdelrehim Khaliland Mostafa Elkhashab

Department of Histology and Cell Biology. Faculty of Medicine, Assiut University, Assiut, Egypt
Department of Medical Physiology. Faculty of Medicine, Assiut University, Assiut, Egypt
Department of Medical Biochemistry. Faculty of Medicine, Assiut University, Assiut, Egypt
Department of Forensic Medicine and Clinical Toxicology. Faculty of Medicine, Assiut University, Assiut, Egypt
Department of plastic surgery .Faculty of Medicine, Assiut University, Assiut, Egypt
Department of Anatomy. Faculty of Medicine, Princess Nourah Bint Abdulrahman University, Riyadh, KSA
Department of Neurosurgery, Hackensack University Medical Center, New Jersey, USA

Posters & Accepted Abstracts: J Tissue Sci Eng

Abstract :

Background: Parkinson's disease is the most common chronic progressive neurodegenerative disorder after Alzheimer's disease. The effectiveness of anti-Parkinson treatments gradually diminished by the progressive degeneration of the dopaminergic terminals .The current research investigated the effect of adipose-derived mesenchymal stem cells (AD-MSCs) versus anti- Parkinson drug in a Parkinsonism rat model. Methods: Forty adult rats divided into 4 equal groups; Group I; control group received the vehicle. Group II; Parkinsonâ??s disease group, received rotenone 2mg/kg daily intraperitoneally for one month. Group III received rotenone at the same previous dose then received isolated AD-MSCs on day 14. Group IV received rotenone at the same previous dose then received carbidopa/levodopa on day 14. Behavioral tests were carried out and midbrain specimens were processed for light and electron microscopy .Genetic expression of Glial fibrillary acidic protein (GFAP) and Nestin mRNA were assessed by Real Time-PCR. Lamin-B1and Vimentin genes were detected by gel electrophoresis and plasma levels of Angiopoietin-2 and Dopamine were measured by ELISA. Results: Rotenone induced pronounced motor deficits, neuronal and glial alterations AD-MSCs group showed improvements in the motor function and microscopic picture .Fold Change of both genes (GFAP and Nestin) were decreased significantly in AD-MSC and carbidopa/levodopa group compared to Parkinsonâ??s disease .Lamin-B1and Vimentin genes were highly expressed in Parkinsonâ??s disease. Plasma levels of Angiopoietin-2 and Dopamine were significantly increased after treatment (P<0.001) compared to Parkinsonâ??s disease. Conclusions: Adipose-Derived-Mesenchymal Stem cells reduced neuronal degeneration more efficiently than the anti- Parkinson drug did in a Parkinsonism rat model.

Biography :

Dr Naglaa Kamal Idriss MBBcH,MSc,MD. Assistant Professor of Medical Biochemistry , Faculty of Medicine ,Assuit . PHD Birmingham University, City hospital, United Kingdom2008, Post doctoral visiting researcher at Southampton University General hospital UK,2016. She has 48 published articles https://www. researchgate.net/profile/Naglaa_Idriss3. She is member for European society of cardiology(ESC) and international stem cell research society(ISSRS), Acute Cardiovascular care association member (ACC), Member of working group of thrombosis (W.G Thrombosis) and Society of heart valve diseases SHVD

E-mail: naglaaidriss@hotmail.com