Biocontrol of macrophomina phaseolina causing charcoal rot of phaseolus vulgaris by pseudomonas sp. PPR8

Joint Webinar: Environmental Microbiology 2022 & 17^th International Conference on Virology, Emerging Diseases & vaccine & 6^th International Conference on Microbes and Beneficial Microbes

November 03, 2022 | Webinar

Pankaj Kumar

Dolphin (PG) Institute of Biomedical and Natural Sciences, India

Scientific Tracks Abstracts: J Antimicrob Agents

Abstract :

Biocontrol of Macrophomina phaseolina was studied using eight isolates of Pseudomonas spp. (PPR1-8). Among the isolate PPR8 showed 65% and 48% reduction in colony growth of the pathogen in dual culture and by CFCF (cell free culture filtrate), respectively. The isolates were fast growers, positive for catalase, oxidase and urease activities and utilized lactose and some amino acids. The isolates are IAA positive, but PPR8 was positive for cyanogens production, solubilization of inorganic and organic phosphate, potassium and zinc, produce siderophore and ACC deamininase. This strain produced extracellular chitinase and β-1,3-glucanase which inhibit the pathogen. 16S DNA of PPR8 was sequenced, analysed and a neighbour-joining dendrogram was generated. Phylogenetic analysis of 16S DNA sequence of PPR8 strain showed maximum sequence similarity with the species of Pseudomonas and recognized as Pseudomonas sp. PPR8 (JN225423). When Pseudomonas sp. PPR8 was used as inoculants in test crops, enhanced germination up to 44% with subsequent increase in grain yield by 27% over control (seeds without inoculums) was recorded. This study reveals the potential of the Pseudomonas sp. PPR8 to be used as a biocontrol agent against M. phaseolina and a good growth promoter for common bean. Key words: Biocontrol, PGPR, Phaseolus vulgaris, Pseudomonas sp. PPR8.

Biography :

Dr. Pankaj Kumar completed his M.Sc. and Ph.D. (Microbiology) from Gurukul Kangri Vishwavidyalaya, Haridwar, Uttarakhand, India. B.Sc. (Botany – Honours) from D.A.V. (PG) College, Siwan, Bihar (Jai Prakash Vishwavidyalaya, Chapra, Bihar, India). Presently working as Assistant Professor in Department of Microbiology, Dolphin (PG) Institute of Biomedical and Natural Sciences, (Affiliated with H.N.B. Garhwal Central University, Srinagar), Dehradun, Uttarakhand, India, Earlier, he served as assistant professor in the Department of MLT, Pt. L.M. S. Govt. (PG) College, Rishikesh, Dehradun, Uttarakhand, India, and in the Department of Microbiology, Dolphin (PG) College of Science & Agriculture Chunni Kalan, Fatehgarh Sahib, Chandigarh, Punjab, India (Affiliated with Punjabi University, Patiala, Punjab, India).