Biomaterial did not cause carcinogenesis in mesenchymal stem cells after various treatments

8^th Euro Global Summit on Cancer Therapy

November 03-05, 2015 Valencia, Spain

Idiberto Jos�© Zotarelli Filho

UNESP University, Brazil

Posters-Accepted Abstracts: J Cancer Sci Ther

Abstract :

Introduction: The identification of chromosomal abnormalities in mesenchymal stem cells from adipose tissue (ADSC) with potential therapeutic use is very important, since these changes may result in carcinogenesis in vivo. The identification of these changes in cells under different conditions is one of the aspects that should be investigated for the realization of cell therapies and slips processes in the safest tissue engineering. Aim: This study aimed to analyze and compare the genetic stability of mesenchymal stem cells in fat after crops matrices chitosancollagen- genipin, cultivation in Petakas and after cryopreservation. Methods: In methodology, samples adipose mesenchymal stem cells taken from fat tissue of 2 female patients undergoing liposuction were studied aged between 30 and 50 years. To study the genetic stability, cells were seeded on chitosan-collagen matrices and genipin-50 cells were examined at 24 h, 48 h and 72 h of culture. We also analyzed 50 cells per number of passes, the first to the tenth passage of the cells in Petakas in order to analyze the cytogenetic stability and all these processes were also repeated after cryopreservation. Results: The results showed that the matrices are biocompatible, exhibit physical and chemical properties needed to house cells in vivo and are strong stimulators of signaling proteins (AP) and other molecules (NO) which are important in tissue healing. Therefore, the matrices provide the biological niche is ADSC adhesion, proliferation and cells agricultural activities. Conclusion: We conclude that the physical and chemical stimuli of scaffolds of collagen-chitosan-genipin did not cause chromosomal abnormalities in adipose mesenchymal stem cells and the biomaterial with great potential for clinical applications.

Biography :

Email: m.zotarelli@gmail.com