Biosensors with nanoscale controlled architecture applicable in glycomics and diagnostics

European Chemistry Congress

June 16-18, 2016 Rome, Italy

Jan Tkac, Tomas Bertok, �½udmila Klukova, Andras Hushegyi, Dominika Pihikova and Stefan Belicky

Slovak Academy of Sciences, Slovakia

Posters & Accepted Abstracts: Chem Sci J

Abstract :

Glycomics is a hot research field since analysis of genomic/proteomic profiles cannot be applied to study many pathological processes. Glycans (complex carbohydrates) are attached to 70% of all proteins and with high density to the surface of the cells. Analysis of protein-linked glycans is crucial for understanding of numerous physiological/pathological processes. Current state-of-the-art tool for glycan analysis is mass spectrometry (MS) combined with chromatographic/electrophoretic instrumentation. MS analysis of some glycan changes associated with cancer is quite challenging due to long analysis time, extensive sample pre-treatment/ derivatization and data interpretation is required by skilled operators. This is why advanced analytical approaches are needed for advancements in the field of glycomics and diagnostics. In this presentation we show that impedimetric biosensors constructed with interfacial layers controlled at nanoscale can detect glycans down to a single molecule level (i.e. aM level). The biosensors were extensively optimised in a way to resist non-specific interactions for analysis of complex samples i.e. serum from patients having various diseases (rheumatoid arthritis, systemic sclerosis, prostate cancer), lysates of isolated human cells (leukaemia) and even with intact leukemic cell lines and flu viruses. Jan.Tkac@savba.sk