Bio-surfactant from Paenibacillus dendritiformis and its application in assisting polycyclic aromatic hydrocarbon (PAH) and motor oil sludge removal from contaminated soil and sand media

3^rd International conference on Bioprocess and Biosystems Engineering

September 14-15, 2015 Baltimore, USA

Fisseha Andualem Bezza and Evans M Nkhalambayausi Chirwa

University of Pretoria, Republic of South Africa

Posters-Accepted Abstracts: J Bioproces Biotechniq

Abstract :

A novel bio-surfactant was produced by strain CN5, identified as Paenibacillus dendritiformis isolated from creosote contaminated wood treatment plant soil. The bio-surfactant produced by the strain was identified as lipopeptidal after Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR), Thin-layer chromatography (TLC), and Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) analysis. The MS/MS analysis of the bio-surfactant showed that it has amino acid sequence of Cys-Gly-Ala-Gly-Ile-Asn-Leu and a long chain fatty acid of molecular mass 522 Da. The biosurfactant exhibited 74% and 82% Emulsification Index (E24) with hexane and cyclohexane, respectively, and it showed high thermal, pH and saline stability over wide range of temperature, pH and salinity. The ability of the bio-surfactant to desorb PAH from spiked soils and motor oil from spiked sands were tested in batch experiments and it desorbed more than 96% of phenanthrene, 83% of pyrene from the contaminated soil in 5 days and 81% of heavy used motor oil sludge from the spiked sands in 24 hrs. This suggests the potential application of the bio-surfactant for the removal of PAHs and motor oil sludge from contaminated media and enhancing their bioremediation by increasing bioavailability and possible application of the bio-surfactant for enhanced oil recovery.

Biography :

Email: fissehaandualem@gmail.com