Carotenoids determination of citruses agro-industrial waste materials using supercritical extraction technique and high performance liquid chromatography

4^th European Chemistry Congress

May 11-13, 2017 Barcelona, Spain

Imeda Rubashvili, Mzia Tsitsagi and Ketevan Ebralidze

Tbilisi State University, Tbilisi, Georgia

Posters & Accepted Abstracts: Chem Sci J

Abstract :

The present research concerns the extraction of carotenoids from Citruses - tangerine and orange agro-industrial waste materials carried out in a dynamic supercritical fluid - carbon dioxide (SC-CO2) extraction system. The carotenoids - beta-carotene and lycopene obtained in organic extracts were quantified using a new, rapid, effective and selective developed and validated HPLC method. The effects of operating pressure and temperature, extraction time, flow rate of the SC-CO2, sample size and solvent nature used were investigated. The optimal conditions for extraction were found. The method was developed using RP-18 endcapped LiChroCART 4 x 250 mm, 5 �¼m column with gradient elution of mobile phase A and B; The method was validated with respect to system suitability test, specificity, linearity-range, accuracy, precision, limit of detection (LOD) and quantitation (LOQ). The stability of solutions were studied as well. The calibration curve is linear over a concentration range 6.497â�� 0.081 �µg/mL for beta-carotene (r2=0.99924) and 18.76 â�� 0.34 �µg/mL for lycopene (r2=0.99990); The LOD and the LOQ are 0.081 �µg/mL and 0.041 �µg/mL for beta-carotene, 0.034 �µg/mL and 0.085 �µg/mL for lycopene, respectively; No interference was observed; The main accuracy is 106.8 % for beta-carotene and 101.4 % for lycopene. The content of each carotenoid per 1 g of dried agro-industrial waste material varies for beta-carotene 0.445 â�� 3.972 �µg (tangerine peel), 0.833 â�� 2.455 �µg (orange peel) and for lycopene 0.051 â�� 179.988 �µg (tangerine peel), 0.091 â�� 0.114 �µg (orange peel). rubashvili@yahoo.fr