Cell affinity chromatography: Experimental study of specific capture mechanisms and implications for industrial development

3^rd International Conference on Medical Physics & Biomedical Engineering

November 07-08, 2016 Barcelona, Spain

Brouchon Julie, Jerome Bibette and Jean Baudry

PSL Research University, France

Posters & Accepted Abstracts: J Bioengineer & Biomedical Sci

Abstract :

Along with the development of cell therapy, cell sorting technology becomes more and more important. Cell specific adhesion is a key phenomenon in affinity cell sorting. We introduce a novel experiment to study the influence of cell velocity on kinetics of specific adhesion of cells. We use a model system: A suspension of biotinylated red blood cells flowing in packed glass beads functionalized with streptavidin (Figure 1). As red blood cell are captured, beads turn red and we can measure cell adhesion kinetics using image analysis of the column (Figure 1) and we determine capture efficacy as a function of cell velocity. We can distinguish two regimes: below 7 mm/s the capture efficacy is greater than 1%, and beyond 7 mm/s capture efficacy greatly decreases to around 0.1%. This study reveals essential results that allow us to design an optimal column and protocol for cell affinity chromatography for cell therapy.

Biography :

Email: jbrouchon@g.harvard.edu