Cellular rejuvenation function of Ginsenoside 20(S)-Rg3 and its ageing-proteome analysis

3^rd International Conference and Exhibition on Traditional & Alternative Medicine

August 03-05, 2015 Birmingham, UK

Su Jeong Baek1, 2, Young-Rang Kim1, Young-Moon Kang1, 2, Kyung-Seo Oh1, Joseph Kwon1 and Jong-Soon Choi1, 2

1Korea Basic Science Institute, Korea 2Chungnam National University, Korea

Posters-Accepted Abstracts: Altern Integr Med

Abstract :

Aging is a multifactorial process resulting from the accumulation of cellular damage over time, leading to physiological deterioration, increased mortality and eventual death. Ginseng is well known in herbal medicine as a tonic and restorative agent. The main molecular ingredients responsible for the actions of ginseng are the ginsenosides (also called ginseng saponins), which are amphiphilic molecules comprising a hydrophobic backbone of aglycone (a hydrophobic four-ring, steroid-like structure) linked to hydrophilic carbohydrate side chains. In previous studies for ginsenoside Rg3, its functions are known to be sodium channel inhibitor in brain disease, anti-angiogenesis effect in diabetic disease, and various anti-cancer activities. However, the effects of ginsenoside Rg3 on the aging/rejuvenation are not reported yet. The senescence associated-�²-galactosidase (SA-�²-gal) activity was dramatically decreased in 20(S)-Rg3-treated human dermal fibroblasts (HDFs) compared to non-treated old HDFs. Moreover, the ginsenoside 20(S)-Rg3 altered numerous aging factors involved in the maintenance of mitochondrial function. To identify the 20(S)-Rg3-induced rejuvenation in HDFs, we analyzed the label-free quantitative proteome in time-dependent proteomic profiles after the treatment of 20(S)-Rg3 to old HDFs. Nano-UPLC-high definition mass spectrometry (HDMSE) revealed the crosstalk with respect to cellular assembly and organization, free radical scavenging and small molecule biochemistry. Among the identified proteins, we concentrated largely in the expression patterns and associated networks of mitochondrial function. It is suggested that the ginsenoside 20(S)-Rg3 can defense aging-associated mitochondrial events and the ginsensoside 20(S)-Rg3 affects the rejuvenation potency by a disclosed molecular mechanism.

Biography :

Email: kyrkhk8@kbsi.re.kr