Characterization of Soil Actinomycete isolate (AI-2) against Gram-positive and Gram-negative food borne bacteria

47^th World Congress on Microbiology

September 10-11, 2018 | London, UK

Amita Gaurav Dimri, Ram Prasad, Abhishek Chauhan, M.L Aggarwal and Ajit Verma

Deaprtment of Microbiology, Shriram Institute for Industrial Research, 19, University Road, Delhi-11007
Amity Institute of Microbial Technology, Amity University, Sector-125, Noida, Uttar Pradesh, India
Amity Institute of Environmental Toxicology, Safety and Management, Amity University, Sector-125, Noida, Uttar Pradesh, India

Posters & Accepted Abstracts: J Med Microb Diagn

Abstract :

Soil microorganisms are the rich source for the isolation and identification of antimicrobial compounds. Among them, actinomycetales are an important group. The present study was performed to isolate actinomycete from soil samples having potential activity against the bacteria isolated from food samples. Five soil samples were collected from different places around Dehradun, Uttarakhand. Potential actinomycete colonies were isolated in pure culture from five soil samples using actinomycete isolation agar. Actinomycete isolate named as AI-2 was identified based on the morphological and biochemical test carried out. A total of ninety one food samples were collected in sterilized container. Fifteen Gram-positive bacteria and twenty gram-negative bacteria were isolated using standard methodology. The bacteria isolated were morphologically and biochemically identified. The Actinomycete isolate (AI-2) was found to have moderate to high activity against fifteen grampositive and twenty gram-negative bacteria. The hexane, ethyl acetate, methanol extracts and mycelium were tested against all bacteria. The ethyl acetate extract showed more potent activity against S. aureus and E .coli. Mycelium of AI-2 isolate did not exhibit any sort of antibacterial activity against any food borne isolates. MIC values of ethyl acetate extract were found in the range of 512-2048 �¼g/ml. Since extract showed inhibitory activity against food borne bacteria, it is suggestive that uttarakhand soil could be an interesting source to explore for antibacterial secondary metabolites.

Biography :

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