Characterization of the carbonic anhydrase 3 gene promoter and the mechanism of Evi1 mediated repression

World Congress on Human Genetics

November 07- 08, 2016 Barcelona, Spain

Alaa Saleh

Glasgow Caledonian University, UK

Posters & Accepted Abstracts: Human Genet Embryol

Abstract :

Ecotropic Viral Integration site-1 (EVI-I) is a transcriptional repressor protein, its expression contributes to acute leukemia and transforms Rat1 fibroblasts cells. Previous microarray studies confirmed that EVI-I is either directly or indirectly regulates transcription of other genes in Rat1 fibroblasts cells and one of these genes is carbonic anhydrase 3 (caIII) which interestingly repressed its expression. In order to understand the mechanism by which EVI-I repress the expression of caIII, the rat caIII promoter region was identified and then serious deletions were conducted. The caIII promoter reporter assay showed that sp1 which located at ��280 caIII promoter region play a major role in regulation of caIII in Rat1 fibroblast cells. Srf, ets and oct which are located at ��137 caIII promoter activity act as potential transcriptional regulation factors may due to combinational activities of all of these three in Rat1 fibroblast cell. For further investigation about the role of these transcription factors on caIII promoter, site direct mutagenesis created with -280 (srf, oct and ets) and reporter assay demonstrates loss of caIII repression as well, which suggesting potential transcription factor located in this area. In order to locate this transcription factor, different deletions created on -280 on caIII gene promoter and reporter assay showed loss of caIII repression at -260 location suggesting that potential c/ebp-e transcription factor may co-operate to activate -280 promoter. These findings might suggest the basis for the development of a novel therapeutic strategy for the treatment of leukemia�s and solid tumors where EVI-I is over-expressed.

Biography :

Alaa Saleh is a currently pursuing PhD at Glasgow Caledonian University specializing in Molecular Biology.

Email: tayeb_alaa@hotmail.com