Cloning and characterization of seed spesific transcription factor for regulating anthocyanin biosynthesis in Indonesia black rice (Oryza sativa ‘Cempo Ireng’)

3^rd International Conference on Genomics & Pharmacogenomics

September 21-23, 2015 San Antonio, USA

Yekti Asih Purwestri

Universitas Gadjah Mada, Indonesia

Posters-Accepted Abstracts: J Mol Genet Med

Abstract :

Black rice is considered as functional food which containing bioactive compounds such as anthocyanin. It is our interest to determine genes responsible for anthocyanin biosynthesis. In this research, we obtained cDNA library from different developmental stages of black rice seeds and white rice seeds as control. Seeds were harvested at different developmental stages (at pollination time, 2 , 7, 14 and 21 days after pollination) for RNA isolation. One �¼g of RNA were subjected for obtaining cDNA library. Semi-quantitative RT-PCR showed that several seed-specifics transcription factors regulating anthocyanin biosynthesis were expressed in Indonesian local black rice. Our previous result showed that two of down regulating genes showed small differences with control rice (white rice cv. Ciherang). Interestingly, one of up-regulating (Os11g059600) demonstrated the high expression level at all four seed developing stages, however only expressed at the day of pollination in white rice control. This result indicated that this gene may play a role in the anthocyanin production during seed development. This gene was reported as conserved hypothetical protein located at chromosome 11 (RAP-DB). The down regulating genes were assumed inhibit anthocyanin metabolism or had a negative effect on the anthocyanin biosynthesis pathway. Further characterization to get insight the function of this gene in the biosynthesis of anthocyanin in black rice cv Cempo ireng was important to develop black rice or other crops as functional food.

Biography :

Email: yektiugm@yahoo.com