Collagen membranes enriched with graphene oxide significantly enhance DPSCs differentiation and control inflammation occurrence

7^th International Conference on Tissue Engineering & Regenerative Medicine

October 02-04, 2017 Barcelona, Spain

Susi Zara, Milena Radunovic, Marianna De Colli, Patrizia De Marco, Chiara Di Nisio, Amelia Cataldi

University â�?�?G. dâ�?�?Annunzioâ�?, Italy
University of Belgrade, Serbia

Posters & Accepted Abstracts: J Tissue Sci Eng

Abstract :

Collagen membranes are used in oral surgical procedures for the treatment of bone defects acting as a barrier that does not allow the invasion of soft tissue into the growing bone. To improve biocompatibility and promote the desired effect on the surrounding tissues, collagen membranes were coated with graphene oxide (GO), a graphene derivative with great potential in bioengineering. The aim of this study was to investigate the biocompatibility of GO coated collagen membranes on human dental pulp stem cells (DPSCs). DPSCs were cultured on uncoated membranes and on both 2 and 10�?¼g/mL GO coated membranes for 3, 7, 14 and 28 days with differentiation medium. Alamar blue and LDH cytotocicity assay were performed, along with PGE2 ELISA assay and real time RT-PCR for RUNX2, BMP2, SP7, TNF�?± and COX2 gene expression. Cellular proliferation is higher on GO coated membranes especially on 10�?µg/ml GO coated one at day 14 and 28. LDH assay evidences no cytotoxic effect, in particular LDH leakage is lower from 10�?µg/ml GO coated membrane at day 14 and 28. BMP2 gene expression is higher in coated membranes at early experimental points whereas RUNX2 and SP7 genes expression appears to be higher on coated membranes at late experimental points. PGE2 secretion levels are lower in GO coated membranes at days 14 and 28. Finally, both TNF�?± and COX2 genes expression are significantly decreased in all experimental points when the highest GO concentration is applied. GO coated membranes are well tolerated by DPSCs, they induce a faster DPSCs differentiation into the osteoblastic lineage and may represent good alternative to conventional membranes thus ensuring more efficient bone formation in guided bone regeneration and improving the clinical performance.

Biography :

Susi Zara graduated in 2006 in Pharmacy at University “G. d’Annunzio of Chieti-Pescara and completed her PhD in 2009 at the same university. She is now a permanent researcher at Pharmacy Department of Chieti’s University. She has published more than 50 papers in international journals. Her fields of research are represented by intracellular signaling in the differentiation of denytla pulp mesenchymal stem cells on innovative biomaterials with a potential use in dental and orthopedic regenerative medicine.