Comparative study between two ex vivo cell expansion models of CD34+ cells from cryopreserved umbilical cord blood co-cultured with encapsulated mesenchymal stem cells

7^th International Conference on Tissue Engineering & Regenerative Medicine

October 02-04, 2017 Barcelona, Spain

Avila-Portillo Luz M, Riveros Angela, Avila Jenniffer, Franco Derly, Ortiz Alejandra, Godoy Marcela and Graciano Noiver

Universidad Militar Nueva Granada, Colombia
CryoHoldco, Colombia
Hospital Militar Central, Colombia

Scientific Tracks Abstracts: J Tissue Sci Eng

Abstract :

The expansion of primitive hematopoietic stem cells (HSCs) with long-term restocking capacity is a technological development necessary to increase the use of umbilical cord blood units in HSC transplantation. At present, CD34+ and CD133+ HSC expansions are reported with adequate expansion factors, however a model is required in which maturation is not induced to compromise lineage progenitors. This work compares the expansion factor (EF) of HSC CD34+/CD90+/CD49f+ using two models of expansion (static and perfusion), the model incorporates mesenchymal cells of Wharton gelatin of CD146+ umbilical cord encapsulated in calcium alginate 2%, and recombinant human cytokines involved in self-renewal (SCF, TOP, FL, IL3, IL6), and the activation of the NOTCH pathway using the DLK-1 agonist in a 12-day culture. NOTCH uses the DLK-1 agonist in a 12-day culture. The perfusion culture vs the static yielded higher EFs for both CD34/45- (8.46 vs. 3.99) and CD49+/CXCR4+HSC (12.97 vs. 2.95) cells. When comparing the two models, it is evident that the perfusion model is statistically significant until day 8 with respect to the static one p<0.005. These results are superior to any study published to date. Comparing the expansion factor in the two culture models, this study suggests the importance of MSC CD146+ in co-cultures with HSC, by sustaining the selective expansion of cd34+/ CD90+CD49f+ without favoring the expansion of other subpopulations of Progenitors taken from unknown lineages. These findings need to be replicated in larger sample sizes in in vivo models and clinical trials of HSC transplantation in humans to demonstrate their importance in important clinical outcomes.