Cytopathological and cytokine expression studies in lymphoma associated with bovine lymphadenopathies

Global Veterinary Microbiology and Veterinary Medicine Summit

October 17-18, 2016 Chicago, USA

Anamika Gupta, Kuldip Gupta, Leishangthem Geeta Devi, N K Sood and Amarjit Singh

Guru AngadDev Veterinary and Animal Sciences University, India

Posters & Accepted Abstracts: J Veterinar Sci Techno

Abstract :

Lymphadenopathies are characteristic features of many pathological conditions. The present study was based on 32 cases of bovine lymphadenopathies, of which, 2 cases of bovine lymphoma were diagnosed on the basis of peripheral blood smear examination and gross lesions in peripheral and visceral lymph nodes and associated organs. Cytology revealed presence of pleomorphic lymphocytes with increased nuclear to cytoplasmic ratio. Histopathological examination in 1st case showed presence of pleomorphic small cell tumor in peripheral lymph nodes and diffuse proliferation of cells with pyknotic nucleus along with secondary bacterial infection in visceral lymph nodes. In 2nd case mediastinal lymph nodes showed presence of monomorphic cell infiltration with thickening of capsule and pleura with infiltration of mixed inflammatory cells and proliferation of lymphoblasts leading to pseudo lobulationin lungs. Immunohistochemistry (IHC) was performed on tissue sections of lymph nodes, lungs, liver and heart by using specific primary antibodies against B-cells (CD20, CD79, p-27) and T-cell (CD3, CD4, CD8). In both cases there were positive cytoplasmic reactivity of CD3 and CD8 with some mild reaction of p27; however, CD20 and CD79 were found negative in both the cases. So, the cases of lymphoblastic lymphadenopathies were diagnosed as T-cell lymphoma. Cytokine expression studies showed significant decrease in IL-2 and IL-4 indicating a decrease inboth the Th1 and Th2 cytokines level which may be associated with the progression of the higher grade of tumor. We conclude from our study that cytopathological and immunohistochemical studies are important for diagnosis of lymphoma associated with bovine lymphadenopathies.

Biography :

Email: vetanamika89@gmail.com