Zahra Hajikarimi, Samide Khoei and S R Mahdavi
Posters-Accepted Abstracts: J Nucl Med Radiat Ther
Objective: Nanotechnology has developed for both cancer diagnosis and therapy. The purpose of this study was to investigate the cytotoxic effect of magnetic nanoparticles as a carrier of 5-fluorouracil and x-ray on the level of proliferation capacity of DU145 prostate carcinoma cell line in monolayer model. Materials and Methods: Therefore, DU145 cells were cultured as monolayer and treated with different concentrations of 5-FU/ or nanoparticles as 5-FU carriers for 24 hours and 2Gy x-ray (6MV). After treatment with nanoparticles, the iron uptake of DU145 cells was monitored using atomic adsorption spectrometry (AAS). The cytotoxic effect of these nanoparticles on the cells was evaluated using the colony formation assay. Results: Our results indicated that iron content and therefore the cellular uptake of 5-FU loaded nanoparticles increased with the increase of nanoparticles concentrations. The viability of the cells is constant as along with the increase of the concentration of free 5-FU and 5-FU encapsulated in nanoparticles. Our finding showed that proliferation capacity of the cells decreased as along with the increase of the concentration of free 5-FU and PLGA coated iron oxide nanoparticles as a carrier of 5-fluorouracil in combination with x-ray. Our results point to the possibility that iron oxide nanoparticles as 5-fu carrier can affect more efficient that 5-Fu as enhanced radiation. Conclusion: According to this study, drug loaded nanoparticles could deliver 5-Fu more efficient into the cells. So, magnetic nanoparticles are effective drug delivery vehicles for 5-FU. PLGA coated iron oxide nanoparticles are biocompatible and this coating is an appropriate surface that can penetrate into the cells.
Nuclear Medicine & Radiation Therapy received 706 citations as per Google Scholar report
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