Development and evaluation of wound healing hydrogel from chicken feather protein

9^th World Convention on Waste Recycling and Reuse

March 11-12, 2019 Singapore

Nilesh M Mahajan

Dadasaheb Balpande College of Pharmacy, India

Keynote: Adv Recycling Waste Manag

Abstract :

Chicken feathers are the largest bio-waste produced across the world. Available uncontrolled disposal methods are not ecofriendly. Chicken feathers consist of about 91% keratin protein. Keratin is a cysteine-rich structural protein with unique characteristics of bioactivity, biocompatibility, biodegradability and natural abundance. Protein component contains a defined three dimensional microstructure that aids in cellular proliferation and cell guided tissue formation. Genistein is a secondary metabolite isoflavones found in soybean. Genistein protects human dermal fibroblasts from oxidative stress-induced collagen biosynthesis inhibition thus acting as an epidermal growth factor. In this research keratin was extracted, purified and characterized to be used in wound healing hydrogel containing genistein. Purified keratin was tested for conformational analysis by FTIR, SEM, SDS-PAGE, amino acid profiling. Extracted keratin was found to retain all the characteristic values with reference standard. The formulated product was then tested for efficacy by in vivo wound healing activity and safety. The optimized wound healing formulation of keratin-genistein gel showed effective results of gel strength, pH, viscosity, spreadability and drug contents. HPTLC study shows presence of both components in the gel without any interactions. Stability study indicates that the formulation was fairly stable. The results of in vivo study indicated that feather keratin hydrogel significantly accelerated the wound healing compared to untreated group. Organ toxicity study reveals safety of developed combination product.

Biography :

Nilesh M Mahajan has completed his PhD from Amravati University, India in Pharmaceutical Sciences. He is currently working as the Head, Department of Pharmaceutics at Dadasaheb Balpande College of Pharmacy, India. He has published 40 papers in reputed journals, granted one international patent. He has design copyright for in vitro dissolution tester.

E-mail: nmmahajan78@gmail.com