Development of a bifunctional PEGylated transferrin-conjugated liposome with gene delivery and tumor targeting properties in vivo

World Congress on Breast Cancer

August 03-05, 2015 Birmingham, UK

Huiqin Zhuo1, Yi Peng2,3, Qin Yao1, Sufang Zhou2, Xi Li2, Xiaoling Lu1,2,3, Yongxiang Zhao2,3

1Xiaman University, China 2Guangxi Medical University, China 3Guangxi Medical University, China 4Guangxi Medical University, China

Posters-Accepted Abstracts: J Cancer Sci Ther

Abstract :

Targeted gene delivery systems that combine imaging and therapeutic modalities in a single macromolecular construct may offer advantages in the development and application of nanomedicines in vivo. Here, we describe the synthesis, biophysical characterization, tumor cell-selective internalization, and targeted gene delivery system of PEGylated transferrin-conjugated liposomes (PTf-Ls). An improved protocol for DNA encapsulation in interstitial space, based on ethanol/calcium-mediated DNA condensation was developed. The nanoparticles obviously accumulated in tumor cells, exhibited high transfection efficiency, which were resistant to serum, and relatively low cell cytotoxicity. In a Balb/c nude mice breast cancer cells MDAMB- 231-Luc (containing luciferase gene) xenograft model, systemic administration of fluorescence dye (Koadak X-sight 670 Large Stokes Shift Dye) labeled PTf-Ls, which effectively eliminated the nonspecific binding, significantly prolonged circulation, and selectively and efficiently internalized in tumor cells, demonstrated a gradual increase of fluorescence in tumor over time up to 26 hrs, specific tumor imaging at 32 h and lasting for more than 58 hrs. Furthermore, we assessed the utility of this system in cancer gene therapy using Interferon (IFN)-�³ inducible protein-10(IP-10) corded plasmid DNA. It dramatically suppressed tumor growth. Therefore, this new bifunctional PTf-Ls system has the potential for gene delivery and targeted tumor imaging in vivo.

Biography :