Diagnosis of cervical cancer and the ecological Papanicolaou: Environmental performance of xylene, hydrochloric acid and ammonia solution

Cancer Diagnostics Conference & Expo

June 13-15, 2016 Rome, Italy

Jeel Moya-Salazar

Universidad Peruana Cayetano Heredia, Peru

Posters & Accepted Abstracts: J Cancer Sci Ther

Abstract :

Little importance has been placed on sustainability and supportability of the Pap stain, standard for the diagnosis of cervical cancer, for the global environmental health, as it uses environmentally toxic and carcinogenic reagents such as xylene, hydrochloric acid and ammonia solution. Here, we propose the eradication of the use of these environmentally toxic-carcinogenic reagents through the validation of the Ecologic Papanicolaou (Eco-Pap). Reagent handling strategies were divided in three phases. We used Harris� progressive Hematoxylin, polychromatic solution and direct mounting that were analyzed by PEED cytology, Staining Quality Index (SQI) and the Bethesda system 2014. A total of 70 603 Pap smear stained with Eco-Pap were admitted (SQI=0.91), validation of Eco-Pap versus the conventional staining for Papanicolaou was optimal (Kappa=0.89) and the sensibility and specificity of the method were of 57% and 98%, respectively, which show a better performance in comparison with the conventional method. Eco-Pap reduced the environmental contamination produced by xylene (72 liters), hydrochloric acid and ammonia (each 6 liters) during one year where all the diagnosis coincided with the cytological and histological details, being cost saving and generating an environmental performance in the diagnosis of cervical cancer. In conclusion, the Eco-Pap is an innovative method that may become into a sustainable and supportable technology.

Biography :

Email: jeelms@upch.pe