Dysregulated expression of dicer in invasive ductal breast carcinoma

9^th Indo Global Summit on Cancer Therapy

November 02-04, 2015 Hyderabad, India

Mohammad Shekari1, Ali Akbar Poursadegh Zonouzi1, Azim Nejatizadeh1, Mohammad Rahmati-Yamchi2, Hedieh Fardmanesh1, Samirashakerizadeh1, Ahmad Poursadegh Zonouzi2 and Kazem Nejati-Koshki2

1Hormozgan University of Medical Sciences, Iran 2Tabriz University of Medical Sciences, Iran

Posters-Accepted Abstracts: J Cancer Sci Ther

Abstract :

Background & Aims: Impaired micro-RNAs processing pathway is one possible mechanism for global down-regulation of the mi-RNAome. Dicer is a key enzyme in mi-RNA processing pathway and dysregulation of its expression has been suggested as a possible cause of mi- RNAome alterations observed in various cancers. However, Dicer mRNA expression in invasive ductal breast carcinoma (IDC) has not been investigated in depth. Therefore, this study aimed to evaluate the mRNA expression of Dicer in IDC and also to assess the correlation of its expression with clinicopathological parameters including age, histological grade, tumor size and lymph node metastasis. Methodology: We investigated the expression of the Dicer in seventy fresh invasive ductal breast carcinomas and matched adjacent nonneoplastic tissue by quantitative real-time PCR using validated reference genes. In addition, the possible impact of clinicopathological characteristics on Dicer expression levels was analyzed. Results: Our results showed that Dicer mRNA expression is down-regulated in slightly more than half (51.43%) of the tumor specimens when compared to adjacent non-neoplastic tissue. Comparison of the Dicer expression level between tumor and matched adjacent non-neoplastic tissue showed that there is no statistical significant differences between them (P=0.425). We also found that Dicer mRNA expression in IDC samples was not correlated with clinicopathological features. Conclusion: Our findings provide additional evidence to support the hypothesis that Dicer expression down-regulated in breast cancer. This study suggested that the decreased expression of Dicer may be potential underlying mechanism in pathogenesis of IDC.

Biography :

Email: mshekari_ch@yahoo.com