Early detection of breast cancer using electrochemical and nano biosensor

Cancer Diagnostics Conference & Expo

June 13-15, 2016 Rome, Italy

Hossein Naderi-Manesh

Tarbiat Modares University, Iran

Posters & Accepted Abstracts: J Cancer Sci Ther

Abstract :

Blood circulating microRNAs are known as novel and validated biomarkers for application in early detection of diseases including cancers. The aim was to design and fabricate precise sensors for quantification of miRNAs in real samples (serum/plasma) without any sample manipulation. Two sensors (an electrochemical biosensor and nanobiosensor) were developed for quantification of miR-155, as a biomarker related to the early phase of breast cancer expression. Both sensors showed high specificity and selectivity, so that they could discriminate between target miR-155 and single-base mismatch, three-base mismatch, completely unmatched oligonucleotides. Reproducibility, stability and functionality of the sensors in the real samples (serum/plasma) were found to be significant. Both sensors had a low detection limit and wide linear ranges with simplicity, less consumed time and low cost, which were superior to the most of previously published works. It seems our method can be introduced to be used in clinical labs and also detection of other miRNA biomarkers through other optimizations.

Biography :

Email: naderman@modares.ac.ir