E-BDSOM-Exopolysaccharide from Bacillus altitudinis, a Deep southern ocean microbe

Annual Industrial Biotechnology and Bioprocessing Congress

September 17-18, 2018 | San Diego, USA

Urmi Halder and Rajib Bandopadhyay

The University of Burdwan, India

Posters & Accepted Abstracts: J Bioprocess Biotech

Abstract :

Exopolysaccharide from Bacillus altitudinis, a Deep Southern Ocean Microbe (E-BDSOM): Microbial EPSs has unique properties, & can be isolated from the bacteria of various ecological niches including marine environment. We have been working on the production & characterization of low molecular weight EPS from Bacillus altitudinis SORB11 isolated from the harsh environment of 3.8 km deep the Southern Ocean, which showed maximum similarity on the basis of 16S rDNA and complete genome analysis with B. altitudinis 41KF2bT, previously reported from 41km above of stratosphere. An important EPS-producing gene region (epsCD) is located in its genome of B. altitudinis. Maximally 11.5 g/L production was obtained & about 70% sugar component of EPS was detected by phenol-H2SO4 estimation. This EPS is porous in nature which is revealed by SEM. The biopolymer existed as flexible chains as well as disordered random inter/intra molecules in water apparently different from the rod-like chains, observed by AFM. XRD was showed the crystalline nature of the EPS. MW was 1.24�103Da determined by GPC. GC-MS showed the presence of mannose & glucose as monomers. Structural characteristics were elucidated by performing FTIR, 1H, 13C including 2D-NMR to detect the presence of the furanoid ring of the sugar units, glycosidic linkage along with the non-carbohydrate parts. Thermostability was also detected & only 45% of the total mass was degraded while the temperature increased up to 600ºC. The viscosity of the EPS was increased by the temperature. The EPS has a good antimicrobial, DPPH & ABTS mediated free radical scavenging activity. Green synthesis of EPS-AgNO3 np-conjugate was prepared and characterized by SEM-EDX, AFM, TEM, FT-IR, UV-Vis spectrum, zeta potential & particle size analyzer. Prepared np-conjugate was round shaped & 10-30μm sized. Antimicrobial activity of the npconjugate was also preferable.

Biography :

E-mail: urmi.microbio@gmail.com