Effect of 3-mercapto-1-hexanol-epigallocatechin-3-gallate -ÃŽÂ²-lactoglobulin nanoparticles hydrocolloids against tumor growth

Qizhen Du; Hao Ying; Chenhuan Yu; Binwu Liao; Zhaoyang Fan; Weihua Zhang; Huazhong Ying; Nagaiya Ravich; ran

doi:10.4172/2472-0542.C1.003

Effect of 3-mercapto-1-hexanol-epigallocatechin-3-gallate -ÃÅ½ÃÂ²-lactoglobulin nanoparticles hydrocolloids against tumor growth

International Conference on Food Chemistry & Hydrocolloids

August 11-12, 2016 Toronto, Canada

Qizhen Du, Hao Ying, Chenhuan Yu, Binwu Liao, Zhaoyang Fan, Weihua Zhang, Huazhong Ying and Nagaiya Ravichandran

Zhejiang A & F University, China
Experimental Animal Center- Zhejiang Academy of Medical Sciences, China

Posters & Accepted Abstracts: J Exp Food Chem

Abstract :

(-)-Epigallocatechin-3-gallate (EGCG), the major catechin in green tea, is primary compound responsible for the health benefits. However, the instability and low bioavailability of EGCG limit its applications. In the present study, EGCG, 3-mercapto-1- hexanol (3MH) and �?²-lactoglobulin was co-assembled into 3MH-EGCG-�?²-LG nanoparticles to improve thermal stability and bioavailability of bioactive EGCG. With a molar ratio of 3MH/EGCG/�?²-LG of 32:32:1, 3MH-EGCG-�?²-LG nanoparticles were prepared with size 31 nm, zeta potential -32.5 mV and PDI 0.083. The nanoparticles showed a sustained release of EGCG in the simulated body fluid condition. 3MH-EGCG-�?²-LG nanoparticles (60 �?¼g EGCG/mL, 3MH/EGCG/�?²-LG=32:32:1) significantly inhibited the growth of human melanoma A375 cells, human hepatoma HepG2 cells and human esophageal cancer TE-1cells. Animal experiments with nude mice bearing transplanted tumor from human melanoma A375 cells gave a tumor growth inhibition rate of 51.7% which was double of those of EGCG alone when the nude mice were treated with the nanoparticles at a equivalent dose of 10 mg EGCG/kg/d by tail intravenous injection lasted 14 days. Enzyme-linked immunosorbent assay showed that 3MH-EGCG-�?²-LG nanoparticles could significantly increase the content of IL-2, TNF-�?± and IFN-�?³, and decrease the content of IL-6 in the mice serum. TUNEL and immunohistochemical detection showed that 3MH-EGCG-�?²-LG nanoparticles could significantly increase the apoptosis index and the activities of caspase-3, and decrease the content of PCNA and VEGF in the tumors. Experimental results also indicated that 3MH-EGCG-�?²-LG nanoparticles could significantly increase the activities of Caspase-3 and Caspase-9, which indicated that 3MH-EGCG-�?²-LG nanoparticles induced the apoptosis of A375 cells via mitochondrial-mediated signaling pathways.