Effect of crocin and doxorubicin/radiation on a breast cancer cell line MCF-7

3^rd World Congress on Women’s Health & Breast Cancer

October 03-05, 2016 London, UK

Vahid Changizi, Ali Reza Fanaie and Majid Safa

Tehran University of Medical Sciences, Iran
Iran University of Medical Sciences, Iran

Posters & Accepted Abstracts: J Cancer Sci Ther

Abstract :

Introduction: Crocin is the main carotenoid of saffron showing anticancer properties. Doxorubicin as a chemotherapy drug and X-ray or gamma radiation therapy are used extensively in the treatment of breast cancer. However, their side effects limited their use. The aim of this study was to investigate the apoptosis of MCF-7 breast cancer cells in monolayer culture (in vitro), using crocin, doxorubicin, radiation, crocin-radiation and crocin-doxorubicin. Materials & Methods: To explore the effect of crocin, doxorubicin and radiation, MCF-7 cell line was cultured and treated with different concentrations of crocin and doxorubicin. MTT assay was used to evaluate the toxicity, PI flowcytometry was used to evaluate the apoptosis and Western blotting was applied to investigate the protein expression of p53, PARP and caspase-7. Results: According to the MTT assay, crocin can decrease growth of MCF-7 cell in a dose and time dependent manner. The results of flowcytometry also showed that apoptosis rate was significantly higher in the combined test than separate tests. Western blot analysis also revealed that the proteins expression in combined groups was much than separated groups. Conclusion: This study revealed the expression of apoptotic proteins in the combined therapy of saffron and radiation or saffron and drug was significantly higher than that in using radiation or drug alone.

Biography :

Email: changizi@sina.tums.ac.ir