Effect of oral supplementation of different Bacillus spp. on growth performance, digestive enzyme activity and immune response in Penaeus vannamei

Proceedings of Microbial Interactions 2021 & Pharmaceutical Microbiology 2021

October 06-07, 2021 WEBINAR

Tamilarasu A*, Ahilan B, Gopalakannan A and Somu Sunder Lingam R

Department of Aquaculture, Dr. M. G. R. FC&RI, Chennai 601 204, Tamil Nadu, India
ICAR-Krishi Vigyan Kendra, Nagapattinam 611 002, Tamil Nadu, India
Krishnagiri-Barur Centre for Sustainable Aquaculture, Tamil Nadu Dr. J.Jayalalithaa Fisheries University, Barur, Krishnagiri 635 201, Tamil Nadu, India

Posters & Accepted Abstracts: JMMD

Abstract :

Bacillus megaterium, B. cereus, B. subtilis, and B. infantis effects in growth and physiological response were assessed on Penaeus vannamei through feed supplementation (108 CFU/g). For this purpose, five treatments were established: control (basal diet without probiotics) and four treatment diets T1 (basal diet + B. megaterium), T2 (basal diet + B. cereus), T3 (basal diet + B. subtilis), T4 (basal diet + B. infantis). During 6 weeks, animals were fed with their respective diets at 10% of body weight, four times a day, and reared in triplicate tanks. Among the treatments, B. subtilis supplemented diet-fed group exhibited significantly higher final weight (0.97 ± 0.06 g), weight gain (0.94 ± 0.06 g), specific growth rate (7.88 ± 0.18 %/day), and survival rate (64.66 ± 4.16%). Similarly, in digestive enzyme activities, the B. subtilis received group showed significantly higher amylase (7.53 ± 1.27 U/mg protein), protease (3.57 ± 0.42 U/mg protein), and lipase (747.83 ± 139.03 U/mg protein) activities compared to other treatments. In contrast, the group that received B. megaterium was higher in cellulase (246.83 ± 29.77 U/mg protein) activity. Interestingly, B. subtilis incorporated diet-fed group exhibits significantly lower activities of phenoloxidase, superoxide dismutase, and catalase. Overall, the study found that the inclusion of B. subtilis improves the growth performance and physiological response of P. vannamei. Before recommending its application in aquaculture practices, further exploration is required on its potentiality in commercial culture tanks. Keywords: Bacillus, digestive enzymes, feed supplementation, growth, probiotic.
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