Effects of ensiled mulberry leaves and sun-dried mulberry fruit pomace on the composition of bacteria in feces of fi nishing steers

3^rd International Conference on Veterinary & Livestock

November 02-03, 2017 Bangkok, Thailand

Yan Li, Qingxiang Meng, Bo Zhou and Zhenming Zhou

State Key Laboratory of Animal Nutrition, China
China Agricultural University, China

Posters & Accepted Abstracts: J Vet Sci Technol

Abstract :

The objective of this study was to compare the eff ects of ensiled mulberry leaves (EML) and sun-dried mulberry fruit pomace (SMFP) on fecal bacterial communities in Simmental crossbred fi nishing steers fed the following 3 diets: A standard TMR diet, standard diet containing EML and standard diet containing SMFP and the diets had similar protein and energy levels. Bacterial communities in the fecal content were analyzed using Illumina Miseq sequencing of the V4 region of the 16S rRNA gene amplifi cation. Quantitative real-time PCR was used to detect the selected bacterial species in the feces. Most of the sequences were assigned to phyla Firmicutes (56.67%) and Bacteroidetes (35.90%), followed by Proteobacteria (1.86%), Verrucomicrobia (1.80%) and Tenericutes (1.37%). And the predominant genera included the 5-7N15 (5.91%), CF231 (2.49%), Oscillospira (2.33%), Paludibacter (1.23%) and Akkermansia (1.11%). As for the treatments, no signifi cant diff erences were observed in Firmicutes (p=0.28), Bacteroidetes (p=0.63), Proteobacteria (p=0.46), Verrucomicrobia (p=0.17) and Tenericutes (p=0.75). On the genus level, classifi ed genera with high abundance (more than 0.1%) mainly came from two phyla: Bacteroidetes and Firmicutes. Also no diff erences were observed in most genera level, 5-7N15 (p=0.21), CF231 (p=0.62), Oscillospira (p=0.9), Paludibacter (p=0.33) and Akkermansia (p=0.37), except that rc4-4 were lower in the CON and SMFP groups compared to the EML animals (p=0.02). Additionally, there were no diff erences in richness estimate and diversity indices (p>0.16), and treatments had no signifi cant eff ect on most selected bacterial species in the fecal (p>0.06), except that Ruminococcus albus were higher in the EML group (p<0.01) and Streptococcus bovis were lower in the CON group (p<0.01). In conclusion, diets supplemented with EML and SMFP have little infl uence on fecal bacterial community composition in fi nishing steers.