Effects of estrogen related receptor inhibition on metabolic adaptations to endurance training in skeletal muscle of male wistar rats

International Conference on Molecular Biology and Medicine

August 27-28, 2018 Dubai, UAE

Soheil Aminizadeh

Kerman University of Medical Sciences, Iran

Posters & Accepted Abstracts: Mol Biol

Abstract :

Fuel consumption is dependent on severity and duration of the exercise. Medium-Chain Acyl-CoA Dehydrogenase (MCAD) and Carnitine Palmitoyl Transferase 1 (CPT1) are involved in this process. MCAD performs the first step in fatty acid oxidation and CPT1 catalyzes the limiting step in mitochondrial beta oxidation. Estrogen-Related Receptor-alpha (ERRα) regulates the genes involved in fatty acid oxidation and mitochondrial biogenesis. The aim of this study was to investigate the effect of 4 weeks of endurance training on the expression of genes involved in lipid metabolism. 30 male wistar rats (8 weeks-old) were randomly divided into four groups: Control (n=7), XCT790 (n=8), trained (n=8) and trained+XCT790 (n=7). Expression of ERRα, MCAD, CPT-1β, PGC-1α, PDK4 and CS genes was measured by Real-Time PCR and quantified by 2-Î?Î?CT method. The expression of ERRα, MCAD, CPT1β, PGC-1α, PDK4 and CS genes were significantly higher in trained compared to control group. The expression of MCAD and CPT1β genes was significantly lower in tained+XCT790 compared trained and the expression of PDK4, CPT1β and PGC-1α were significantly higher in Trained+XCT790 compared to control+XCT790. Taken together, expression of ERRα is a trainable factor and its changes are parallel with higher expression of the enzymes which involved in lipid metabolism. This indirectly suggests a significant role of ERRα in adaptation of lipid metabolism evoked by endurance training.

Biography :

E-mail: soheilaminizadeh@yahoo.com