Effects of onion (Allium cepa) and chloramphenicol on haematological parameters, histopathology and survival of catfish Clarias gariepinus (Burchell, 1822) sub-adult infected with Pseudomonas aeruginosa

Global Veterinary Summit

August 31- September 02, 2015 Orlando-FL, USA

Adeoye Joshua Oyewusi, Amrevuawho Mavis Oghenebrorhie, Ezeri Godfrey NnamdiOnyenoro, Akinyemi Adeolu Akanji and Olufunmilayo Mobolaji Bankole

Federal university of Agriculture, Nigeria

Posters-Accepted Abstracts: J Veterinar Sci Technolo

Abstract :

The study was carried out to evaluate the antimicrobial characteristics, immunostimulant ability and survival of P. aeruginosa infected Clariasga riepinus sub-adult exposed to chloramphenicol and Allium cepa (onion). The antibacterial efficacy of chloramphenicol and onion extract was screened against type culture of P. aeruginosa ATCC 27853 at 100%, 75% and 50% concentration using agar well diffusion method. Minimum inhibitory concentration (MIC) was determined using microtube broth dilution method and minimum bactericidal concentration (MBC) was also determined. P. aeruginosa infected fish were exposed to chloramphenicol and onion in vivo in prolonged bath treatment twice daily for 7 days. A total of six (6) experimental fish from the treatments were tested for their cellular immune response to P. aeruginosa and to the different treatment. Histological changes were evaluated before, after challenge and after treatment. Percentage survival was calculated by recording number of mortality. There was no significant (p>0.05) difference in their susceptibility to the test bacteria. However, MIC for chloramphenicol and onion were greater than 50 mg/ml and MBC of 50 mg/ml was only obtained for onion. Haematological values of infected fish revealed significant (p>0.05) decrease in parked cell volume (PVC, 22.33�±0.3), haemoglobin (HB, 6.97�±0.2), red blood cell (RBC, 1.27�±0.1), lymphocyte (52.33�±0.9) and significant (p>0.05) increase in white blood cell (WBC, 17.13�±0.5), neutrophil (47.33�±1.2) than that of control fish with PCV (34.67�±5.2), HB (9.77�±0.2), RBC (2.23�±0.3), lymphocyte (69.00�±2.3) and WBC (10.80�±0.3), neutrophil (30.33�±2.9) but no significant (p>0.05) changes were observed in all blood parameters among infected fish treated with chloramphenicol and A. cepa. Photomicrograph of damaged organs (gills, liver and stomach) showed moderate regeneration of organs exposed to chlorampenicol and onion. Percentage survival was high in infected and treated experimental fish. Antibacterial potentials of onion can therefore be exploited as alternative in combating infections of P. aeruginosa in fish.

Biography :

Email: