Effects of reduced glutathione, water soluble vitamin E analogue and butylated hydroxytoluene on quality of cryopreserved boar spermatozoa

International Conference and Exhibition on Tissue Preservation & Bio-banking

July 20-22, 2015 Barcelona, Spain

Santosh Kumar Baishya1, R K Biswas2, G Kadirvel1, B C Deka2 and Suresh Kumar1

Posters-Accepted Abstracts: J Tissue Sci Eng

Abstract :

The objective of the present study was to evaluate the comparative effects of reduced glutathione (GSH), water soluble Vitamin E analogue Trolox and butylated hydroxytoluene (BHT) on quality of cryopreserved boar spermatozoa. Sixteen sperm-rich fractions of ejaculates, collected from six mature boars were utilized for the study. Using split sample technique three different antioxidants viz., GSH (1 mM), Vitamin E (0.2 mM) and BHT (0.2 mM) were added to the freezing medium of lactose-egg yolkglycerol extender and frozen using controlled freezing rate of 40Ë?C/min from -6 to -140Ë?C in medium straw. Semen samples were evaluated for sperm motility, acrosomal status, plasma membrane integrity, mitochondrial membrane potential (MMP), lipid peroxidation (LPO) and sperm DNA integrity after equilibration and after freezing. Results revealed that GSH, Vitamin E and BHT addition resulted in significantly (p<0.05) higher post thaw motility, live intact acrosome and plasma membrane intact sperm as compared to no supplementation (control). The incidence of post thaw sperm lipid peroxidation was also significantly (p<0.05) reduced after supplementation of antioxidants. However, antioxidants treatment neither significantly (p>0.05) improved MMP of live sperm sub-population nor sperm DNA integrity after freezing. In addition, there was no significant difference of the post thaw sperm characteristics among the three antioxidants. Our findings suggest that the magnitude of protective measure conferred by GSH, Vitamin E and BHT on cryopreserved boar spermatozoa are comparable and thus incorporation of either GSH, Vitamin E or BHT in freezing medium could provide boar semen with better freezability.