Enhanced therapeutic effect of Adriamycin on multidrug resistant breast cancer by the ABCG2- siRNA loaded polymeric nanoparticles assisted with ultrasound

3^rd World Congress on Women’s Health & Breast Cancer

October 03-05, 2016 London, UK

Lianfang Du, Min Bai, Fan Li, Yang Liu and Yongrong Duan

Shanghai General Hospital- Shanghai Jiao Tong University, Shanghai, China
Renji Hospital, School of Medicine- Shanghai Jiao Tong University, China

Posters & Accepted Abstracts: J Cancer Sci Ther

Abstract :

The overexpression of the breast cancer resistance protein (ABCG2) confers resistance to Adriamycin (ADR) in breast cancer. The silencing of ABCG2 using small interfering RNA (siRNA) could be a promising approach to overcome multidrug resistance (MDR) in cancer cells. To deliver ABCG2-siRNA effectively into breast cancer cells, we used mPEG-PLGA-PLL (PEAL) nanoparticles (NPs) with ultrasound-targeted micro-bubble destruction (UTMD). PEAL NPs were prepared with an emulsion-solvent evaporation method. The NPs size was about 131.5�±6.5 nm. The siRNA stability in serum was enhanced. The intracellular ADR concentration increased after the introduction of siRNA-loaded NPs. After intravenous injection of PEAL NPs in tumor-bearing mice, the ABCG2-siRNA-loaded NPs with UTMD efficiently silenced the ABCG2 gene and enhanced the ADR susceptibility of MCF-7/ADR (ADR resistant human breast cancer cells). The siRNA-loaded NPs with UTMD+ADR showed better tumor inhibition effect and good safety in vivo. These results indicate that ADR-chemotherapy in combination with ABCG2-siRNA is an attractive strategy to treat breast cancer.

Biography :

Email: medicineli@163.com