Estimation of caffeine in different beverages by ultraviolet spectroscopySumit Kumar, Ashish Baldi, Deepika Tanwar and Pramod Patwal

2^nd International Conference and Exhibition on Pharmaceutical Regulatory Affairs

November 23-24, 2012 Hyderabad International Convention Centre, India

Senthil Balan S and Jayalakshmi S

Accepted Abstracts: Pharmaceut Reg Affairs

Abstract :

This study was undertaken with the objective of estimation the concentration of caffeine in seven brands of soft drink with the use of an analytical method. Caffeine is a central nervous system (CNS) stimulant and a bitter white crystalline xanthine alkaloid. Preparation: The different brands of beverages were procured from different companies like Red Bull, Pepsi, Cola-Cola, Mountain dew, XXX, Power-X, and Thumps-up. The caffeine content in the different beverages was determined by dilution by water and then absorbance was measured by U.V Spectrophotometer Shimanzu1800 compact by absorption at λ max 271.2 nm. The amount of caffeine was determined against the absorbance from the standard curve of caffeine in distilled water at same λ max in the range of 5-30 μg/ml. The highest concentration of caffeine was found in Power-X (46μg/ml), and remaining was found to be containing 44μg/ml to 20.5μg/ml. So Power-X could be called as a strongest CNS stimulant among all samples and it could be harmful for health. And the lowest concentration of caffeine was found in XXX (19.5μg/ml) so it could be weaker CNS stimulant among all samples.

Biography :

S.Mounika has completed her graduation in Biotechnology and doing masters in Bioinformatics from Amity Institute of Biotechnology,Noida. She have attended one poster presentation and participated in national science congress and AP science congress,and won first prize in oral presentation.