Evaluating whether radiofrequency irradiation attenuated UV-B-induced skin pigmentation by increasing melanosomal autophagy and decreasing melanin synthesis

23^rd World Dermatology Congress

June 20-21, 2022 | Paris, France

Hyoung Moon Kim

Maylin Clinic, South Korea

Keynote: J Dermatol Dis

Abstract :

Autophagy is involved in the degradation of melanosomes and the determination of skin color. TLR4 and tumor necrosis factor (TNF) signaling upregulates NF-κB expression, which is involved in the upregulation of mTOR. The activation of mTOR by UV-B exposure results in decreased autophagy, whereas radiofrequency (RF) irradiation decreases TLR4 and TNF receptor (TNFR) expression. We evaluated whether RF decreased skin pigmentation by restoring autophagy by decreasing the expression of TLR4 or TNFR/ NF- κB /mTOR in the UV-B-irradiated animal model. UV-B radiation induced the expressions of TNFR, TLR, and NF-κB in the skin, which were all decreased by RF irradiation. RF irradiation also decreased phosphorylated mTOR expression and upregulated autophagy initiation factors such as FIP200, ULK1, ULK2, ATG13, and ATG101 in the UV-B-irradiated skin. Beclin 1 expression and the expression ratio of LC3-I to LC3-II were increased by UV-B/RF irradiation. Furthermore, melanin-containing autophagosomes increased with RF irradiation. Fontana-Masson staining showed that the amount of melanin deposition in the skin was decreased by RF irradiation. This study showed that RF irradiation decreased skin pigmentation by restoring melanosomal autophagy, and that the possible signal pathways which modulate autophagy could be TLR4, TNFR, NF-κB, and mTOR.

Biography :

Hyoung Moon Kim has his expertise in microbiology, having completed his master’s degree in microbiology at Chungnam National University. His passion lies in enhancing the health and natural beauty of people. From 2018 to 2021, he served as the president of KALDAT(Korean Association for Laser, Dermatology and Trichology) taking further steps to accomplish his goal. He has had more than ten papers published in reputed journals, and now serving as the editor-in-chief of “The Aesthetics” journal.