Evaluation the effect of GABA in the proliferation and migration of fibroblasts in vitro

5^th International Conference and Exhibition on Pharmaceutical Regulatory Affairs

August 03-05, 2015 Orlando, USA

Lucimar Filot da Silva Brum, Vani dos Santos Laranjeiraa and Ivana Grivicich

Lutheran University of Brazil, Brazil

Posters-Accepted Abstracts: Pharmaceut Reg Affairs

Abstract :

Skin aging characterized by sagging, blemishes and wrinkles, resulting from tissue structural changes, especially due to decrease in assets fibroblasts and consequent decrease in elasticity and collagen synthesis. It is a high demand for active compounds that may be incorporated into cosmetics for preventing and slowing the signs of skin aging. Growth factors or analogous substances such as, Carboxyethyl Gamma Aminobutyric Acid (CEGABA), a neuromodulator derived from polyamines appears to be a promising substance in the rejuvenation strategies. However, there are no studies proving its effectiveness and safety. In this context, the present study was to evaluate the effect of CEGABA in the proliferation and migration of fibroblasts in vitro. We used a fibroblast cell line NIH 3T3, which was exposed to CEGABA compound and the analysis of cell proliferation was performed by inoculating the cells in plaques and assessment of cell migration in vitro, we used the scratch wound assay. Cytotoxicity assessed using the MTT colorimetric assay, and assessing the genotoxicity performed using the alkaline comet assay version. Our results indicated that the compound CEGABA stimulated cell proliferation and migration of fibroblasts and did not show cytotoxic and genotoxic effects, suggesting that the use of CEGABA in cosmeceuticals is safe and can be used as adjuvant in therapeutic against aging.

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