Expression profile of HOXA 6-13 genes in women with polycystic ovaries

3^rd World Congress on Human Genetics and Genetic Disorders

October 20-21, 2017 | Toronto, Canada

Soltani-Bayat Mehri, Momeni-moghadam Madjid, Favaedi Raha, Eftekhari Yazdi Poopak and Shahhoseini Maryam

Hakim Sabzevari University, Iran
Royan Institute for Reproductive Biomedicine, Iran

Scientific Tracks Abstracts: J Mol Genet Med

Abstract :

Statement of the Problem: Polycystic ovarian syndrome (PCOS) as an ovarian disorder and multifactorial disease involve 6-15% of women in the fertility age. Probably, some factors effect on the follicogenesis, so were caused PCOD (polycystic ovary diagnosis). Genetic and environmental agents are of main factors in this regard. Genetic polymorphisms or mutations, X-linked and autosomal dominant inheritance, paternal metabolic syndrome and altering gene expression are example of genetic factors. Deregulation of HOX genes in various diseases was studied by scientists. HOX family genes code transcription factors that influence transcript target genes. Some studies have confirmed the role of these genes in follicogenesis and reproductive system evolution. Cumulus cells encircle the oocyte and can influence in the function of oocyte through cell-to-cell communication. The aim of this study was to focus on the expression pattern of HOXA family genes in cumulus oophorus in women with PCOS and PCOD vs. control (healthy samples). Methodology & Theoretical Orientation: Cumulus cells ware extracted from women with PCOS and PCOD and normal women (infertile men�s wife). RNA was extracted from these cells, and cDNA was synthesized consequently. The expression of HOXA genes were checked with real-time PCR using designed primers. Data were analyzed with SPSS. Findings: As shown in the chart, we observed increased expression of HOXA9, 13 in PCOS vs. PCOD and control; increased expression of HOXA6 in PCOS and PCOD vs control, and increased expression of HOXA7, 10, 11 in PCOS women vs. control. Conclusion & Significance: We found hyper expression of HOXA genes in women with polycystic ovaries. Thus the regulation of expression of these genes can be considered in improvement of women diagnosed with PCO.

Biography :

Soltani-Bayat Mehri is pursuing her MSc in Molecular-Cellular Biology at Hakim Sabzevari University. She is working on her thesis about gene expression in PCOS patient in Royan Institute.