Fabrication of herbal scaffold for bone tissue engineering using Eucomis autumnalis plant extract

Annual Conference on Bioscience

September 12-13, 2016 Berlin, Germany

Franca Nneka Alaribe and Motaung S C K M

Tshwane University of Technology, South Africa

Posters & Accepted Abstracts: J Bioeng Biomed Sci

Abstract :

In spite of the frequent use of different biomaterials as a substrate for seeded cells and for supports of the formation of new matrix deposition, little or no work has been done in incorporating medicinal plants extracts with anti-inflammatory and osteoinductive capacity in scaffold fabrication, hence, our focus on a novel herbal scaffold incorporating medicinal plant Eucomis autumnalis extract with natural biopolymers, alginate and chitosan by lyophilization technique. The prepared composite scaffolds with plant extract were further characterized using X-ray diffraction analysis (XRD), Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR).Our data showed that the formed scaffold is an ideal scaffold. Toxicity, compatibility, differentiation capacity, alkaline phosphate performance, SEM, inflammatory activity using Cyclooxygenase enzyme (COX-1, COX-2) activity of the fabricated scaffold were investigated on porcine derived adipose stem cells (pASCs). The XRD and Ft-IR tests different peaks indicated clear presence of CaCO3, HPO4 and H2O compounds. The 3-(4,5-dimethythiazol2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay in cells showed the herbal scaffold to be non-toxic, increasing proliferation with cell viability more than scaffolds without herbal extract, swelling ratio for water uptake showed higher in scaffolds incorporated with the plant extract. The herbal scaffolds were found to selectively inhibit COX-2 expression in cells. The physiochemical and biological properties of the herbal scaffold showed osteo-inductive support to pASCs. E. autumnalis extract in scaffolds may provide safe and cost effective alternative treatment for bone fracture and regeneration, reducing the long hospitalization of patients with bone defects and other bone therapeutics.

Biography :

Email: FrancaN@tut.ac.za